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Cell-Division-Cycle Kinase 2 (cdc2) antibody

Antigen

Cell-Division-Cycle Kinase 2 (cdc2)
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649515
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Cdc2
Gene ID 983
UniProt P06493
Description Other names: CDC2, Cell division control protein 2 homolog, p34 protein kinase,Cyclin-dependent kinase 1
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues of human Cdc2 was used as immunogen. Predicted to cross-react with most species, based on sequence homology.
Molecular Weight 35 kDA
Comments

Background: During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:40
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Cdc2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Norbury, Nurse: "Animal cell cycles and their control." in: Annual review of biochemistry, Vol. 61, pp. 441-70, 1992 (PubMed).

Watanabe, Broome, Hunter: "Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle." in: The EMBO journal, Vol. 14, Issue 9, pp. 1878-91, 1995 (PubMed).

Galaktionov, Jessus, Beach: "Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation." in: Genes & development, Vol. 9, Issue 9, pp. 1046-58, 1995 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Cell-Division-Cycle Kinase 2 (cdc2)", type "Antibodies"
Hosts Rabbit (70), Mouse (10), Goat (1)
Reactivities Human (75), Rat (Rattus) (58), Mouse (Murine) (48), Chicken (11), Cow (Bovine) (11), Horse (Equine) (10), Pig (Porcine) (10), Sheep (Ovine) (10), Dog (Canine) (6), Arabidopsis thaliana (1), Barley (Hordeum vulgare) (1), Cat (Feline) (1), Maize/Corn (Zea mays) (1), Rice (Oryza sativa) (1), Xenopus laevis (1)
Applications Western Blotting (WB) (50), Immunofluorescence (IF) (32), Immunohistochemistry (IHC) (27), ELISA (18), Flow Cytometry (FACS) (4), Immunoprecipitation (IP) (3), Immunocytochemistry (ICC) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Dot Blot (Dot) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4)
Epitopes pTyr15 (15), pThr161 (12), pThr14 (5), pSer39 (4), Thr161 (2), Tyr15 (2), C-Term (1), N-Term (1), pSer54 (1), pTyr14 (1), pTyr774 (1)