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Details for Product No. ABIN649516

Cyclin-Dependent Kinase 1 (CDK1) antibody

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Antigen
Synonyms
CDC2, CDC28A, P34CDC2, Cdc2, Cdc2a, p34, cdc2, wu:fc30e01, zgc:92032, 5363, CDCDm, CDK1, CDK1/CDC2, CG5363, Cdk-1, Cdk1, Dcdc2, Dm cdc2, DmCdc2, DmCdk1, Dmcdc2, Dmel\\CG5363, cdc, cdc2Dm, cdk1,  ... show more
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 3 references available
Catalog no. ABIN649516
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues surrounding Tyr15 of human Cdc2 was used as immunogen. The antibody detects Cdc2 without phosphorylation on Tyrosine 15. It does not detect Y15-phosphorylated Cdc2. Predicted to cross-react with bovine, based on sequence homology.
Alternative Name Cdc2
Background During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).
Synonyms: CDC2, Cell division control protein 2 homolog, p34 protein kinase,Cyclin-dependent kinase 1
Molecular Weight 34 kDA
Gene ID 983
UniProt P06493
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:50 ICC: ~~ 1:500 IHC: ~~ 1:50
Comment

Background: During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Cdc2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Norbury, Nurse: "Animal cell cycles and their control." in: Annual review of biochemistry, Vol. 61, pp. 441-70, 1992 (PubMed).

Watanabe, Broome, Hunter: "Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle." in: The EMBO journal, Vol. 14, Issue 9, pp. 1878-91, 1995 (PubMed).

Galaktionov, Jessus, Beach: "Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation." in: Genes & development, Vol. 9, Issue 9, pp. 1046-58, 1995 (PubMed).

Alternatives for antigen "Cyclin-Dependent Kinase 1 (CDK1)", type "Antibodies"
Hosts (337), (129)
Reactivities (420), (248), (235), (45), (38), (31), (27), (26), (14), (9), (7), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applications (376), (158), (129), (83), (72), (60), (53), (32), (15), (11), (11), (11), (7), (4), (3), (3), (1), (1), (1), (1)
Conjugates (13), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Epitopes (50), (49), (45), (24), (17), (15), (10), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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