Cyclin-Dependent Kinase 1 (CDK1) antibody

Details for Product No. ABIN649517
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Antigen
Reactivity
Human
(637), (279), (257), (37), (14), (12), (10), (7), (6), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(541), (143)
Clonality
Monoclonal
Conjugate
Un-conjugated
(32), (25), (22), (17), (16), (16), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(567), (250), (181), (110), (96), (80), (60), (38), (36), (24), (17), (12), (10), (8), (7), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Request Want additional data for this product?

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Specificity A synthetic peptide corresponding to residues near the C-terminus of human Cdc2 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.
Alternative Name Cdc2 (CDK1 Antibody Abstract)
Background During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).
Synonyms: RPS6KA3, ISPK1, RSK2, Ribosomal protein S6 kinase alpha-3, MAP kinase-activated protein kinase 1b
Molecular Weight 34 kDA
Gene ID 983
UniProt P06493
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure
Application Notes WB: = 1:500-2000
Comment

Background: During the eukaryotic cell cycle, activation of Cdc2 (Cdk1) kinase regulates the transition into M-phase (1). Cdc2 forms a complex with cyclin B, and activation of this complex is controlled by dephosphorylation of Cdc2 at tyrosine 15 and threonine 14 (2). Phosphorylation at Tyr15 and is carried out by Wee1 and Myt1 protein kinases (3), while Tyr15 dephosphorylation is carried out by the cdc25 phosphatase (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Cdc2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Galaktionov, Jessus, Beach: "Raf1 interaction with Cdc25 phosphatase ties mitogenic signal transduction to cell cycle activation." in: Genes & development, Vol. 9, Issue 9, pp. 1046-58, 1995 (PubMed).

Watanabe, Broome, Hunter: "Regulation of the human WEE1Hu CDK tyrosine 15-kinase during the cell cycle." in: The EMBO journal, Vol. 14, Issue 9, pp. 1878-91, 1995 (PubMed).

Norbury, Nurse: "Animal cell cycles and their control." in: Annual review of biochemistry, Vol. 61, pp. 441-70, 1992 (PubMed).

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Catalog No. ABIN649517
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