Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibody

Antigen

Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C)
Synonyms Cdc25, CDC25C, CDC25, cdc25, xcdc25c
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649518
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives back to top

Alternative name Cdc25C
Gene ID 995
UniProt P30307
Description Other names: CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2, 4 and 5 of human Cdc25C, based on sequence homology.
Molecular Weight 60 kDA
Comments

Background: Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5).

Application Details
Publications Alternatives back to top

Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:250 IHC: ~~ 1:250~500
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Cdc25C Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Metabolism, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Sadhu, Reed, Richardson et al.: "Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 13, pp. 5139-43, 1990 (PubMed).

Peng, Graves, Thoma et al.: "Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1501-5, 1997 (PubMed).

Jessus, Ozon: "Function and regulation of cdc25 protein phosphate through mitosis and meiosis." in: Progress in cell cycle research, Vol. 1, pp. 215-28, 1998 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C)", type "Antibodies"
Hosts Rabbit (75), Mouse (29)
Reactivities Human (102), Mouse (Murine) (32), Rat (Rattus) (31), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)
Applications Western Blotting (WB) (66), Immunofluorescence (IF) (49), ELISA (33), Immunohistochemistry (IHC) (24), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunoprecipitation (IP) (19), Immunocytochemistry (ICC) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (3), Alexa Fluor 488 (3), Alexa Fluor 555 (3), Alexa Fluor 647 (3), PE,Cy5.5 (2), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes pSer216 (16), pThr48 (6), C-Term (4), N-Term (4), Internal Region (2), Ser216 (2), pSer198 (2), AA 1-258 (1), Center (1), pSer178 (1), pThr161 (1)