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Details for Product No. ABIN649518

Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C) antibody

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Antigen
Synonyms Cdc25, CDC25, PPP1R60, cdc25, cdc25-3, xcdc25-3, xcdc25c, CDC25C
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 3 references available
Catalog no. ABIN649518
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2, 4 and 5 of human Cdc25C, based on sequence homology.
Alternative Name Cdc25C
Background Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5).
Synonyms: CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C
Molecular Weight 60 kDA
Gene ID 995
UniProt P30307
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Metabolism, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:250 IHC: ~~ 1:250~500
Comment

Background: Cdc25C is a tyrosine protein phosphatase required for progression of the cell cycle. Cdc25C activates cell cycle-specific cyclin-dependent kinases (CDKs), including cdc2, via dephosphorylation events (1,2). Cdc25C is activated via phosphorylation at Ser216 (3). In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (4,5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Cdc25C Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sadhu, Reed, Richardson et al.: "Human homolog of fission yeast cdc25 mitotic inducer is predominantly expressed in G2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 13, pp. 5139-43, 1990 (PubMed).

Peng, Graves, Thoma et al.: "Mitotic and G2 checkpoint control: regulation of 14-3-3 protein binding by phosphorylation of Cdc25C on serine-216." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1501-5, 1997 (PubMed).

Jessus, Ozon: "Function and regulation of cdc25 protein phosphate through mitosis and meiosis." in: Progress in cell cycle research, Vol. 1, pp. 215-28, 1998 (PubMed).

Alternatives for antigen "Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C)", type "Antibodies"
Hosts (120), (60), (1)
Reactivities (181), (35), (32), (12), (1), (1), (1), (1)
Applications (124), (55), (46), (38), (36), (31), (30), (12), (2), (2), (2), (2), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Epitopes (35), (17), (8), (8), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
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