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Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (pSer106) antibody

Details for Product No. ABIN649521
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Antigen
Synonyms CDC18L, HsCDC18, HsCDC6
Epitope
pSer106
(23), (10), (6), (6), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(124), (41), (30), (12), (12), (12), (2), (1), (1)
Host
Rabbit
(92), (32), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(8), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB)
(93), (44), (33), (20), (19), (18), (10), (3), (2), (2), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN649521
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Specificity A phospho-specific peptide corresponding to residues surrounding Serine 106 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 106.
Alternative Name Cdc6
Background The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4).
Synonyms: CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein,p62(cdc6)
Molecular Weight 62 kDA
Gene ID 990
UniProt Q99741
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000
Comment

Background: The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Cdc6 Antibody Phospho (pS106) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Williams, Shohet, Stillman: "A human protein related to yeast Cdc6p." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 142-7, 1997 (PubMed).

Yan, DeGregori, Shohet et al.: "Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3603-8, 1998 (PubMed).

Jiang, Wells, Hunter: "Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 11, pp. 6193-8, 1999 (PubMed).

Hall, Kow, Nevis et al.: "Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3340-50, 2007 (PubMed).

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