Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (pSer106) antibody

Antigen: Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6)

Synonyms: CDC18L, HsCDC6, HsCDC18

Binding Site: pSer106

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN649521

Additional Information

Alternative name: Cdc6

Gene ID: 990

UniProt: Q99741

Description: Other names: CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein,p62(cdc6)

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho-specific peptide corresponding to residues surrounding Serine 106 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 106.

Molecular Weight: 62 kDA

Comments:

Background: The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Cdc6 Antibody Phospho (pS106) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Williams, Shohet, Stillman: "A human protein related to yeast Cdc6p." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 142-7, 1997 (PubMed).

Yan, DeGregori, Shohet et al.: "Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3603-8, 1998 (PubMed).

Jiang, Wells, Hunter: "Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 11, pp. 6193-8, 1999 (PubMed).

Hall, Kow, Nevis et al.: "Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3340-50, 2007 (PubMed).