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Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (pSer106) antibody
|Synonyms||CDC18L, HsCDC6, HsCDC18|
Alternatives Western Blotting (WB)
|4 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein,p62(cdc6)|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A phospho-specific peptide corresponding to residues surrounding Serine 106 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 106.|
|Molecular Weight||62 kDA|
Background: The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6 participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). In vitro, Cdc6 is an excellent substrate for Cdk2, phosphorylated at three sites (Serine 54, Serine 74 and Serine 106) (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~2000|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Cdc6 Antibody Phospho (pS106) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Williams, Shohet, Stillman: "A human protein related to yeast Cdc6p." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 142-7, 1997 (PubMed).
Yan, DeGregori, Shohet et al.: "Cdc6 is regulated by E2F and is essential for DNA replication in mammalian cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3603-8, 1998 (PubMed).
Jiang, Wells, Hunter: "Multistep regulation of DNA replication by Cdk phosphorylation of HsCdc6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 11, pp. 6193-8, 1999 (PubMed).
Hall, Kow, Nevis et al.: "Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3340-50, 2007 (PubMed).
|Hosts||Rabbit (46), Mouse (19)|
|Reactivities||Human (64), Mouse (Murine) (32), Rat (Rattus) (28), Cow (Bovine) (19), Dog (Canine) (6), Horse (Equine) (5), Pig (Porcine) (5), Sheep (Ovine) (5), Primate (2), Cat (Feline) (1), Chicken (1), Yeast (Saccharomyces cerevisiae) (1)|
|Applications||Western Blotting (WB) (44), Immunofluorescence (IF) (24), ELISA (20), Immunoprecipitation (IP) (14), Immunohistochemistry (IHC) (9), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Biotin (4), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), PE,Cy5.5 (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)|
|Epitopes||pSer54 (8), N-Term (3), AA 400-500 (1), C-Term (1), pSer353 (1)|