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Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6) (pSer54) antibody

Details for Product No. ABIN649522
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Antigen
Synonyms CDC18L, HsCDC6, HsCDC18
Epitope
pSer54
(21), (10), (6), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(123), (40), (30), (12), (12), (12), (2), (1)
Host
Rabbit
(90), (33), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(8), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(92), (47), (30), (20), (19), (18), (7), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN649522
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A phospho-specific peptide corresponding to residues surrounding serine 54 of human Cdc6 was used as an immunogen. The antibody only detects Cdc6 phosphorylated on Serine 54.
Alternative Name Cdc6
Background The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6p and cdc18+ are unstable proteins that are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. They participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). Cdc6 is believed to be phosphorylated at Serine 54 during S phase and functions as a chromatin-bound signal that prevents reformation of prereplication complexes (4).
Synonyms: CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein,p62(cdc6)
Molecular Weight 62 kDA
Gene ID 990
UniProt Q99741
Research Area Phospho-specific antibodies, Protein Modifications, Cell Cycle, Signaling, Chromatin, Cell Structure
Application Notes WB: = 1:1000-2000
Comment

Background: The Cdc6 protein is an essential component of pre-replication complexes (preRCs), which assemble at origins of DNA replication during the G1 phase of the cell cycle (1). Cdc6p and cdc18+ are unstable proteins that are essential and rate limiting for the initiation of DNA replication in Saccharomyces cerevisiae and Schizosaccharomyces pombe, respectively. They participate in checkpoint controls that ensure DNA replication is completed before mitosis is initiated (2). It has been concluded that expression of Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells (3). Cdc6 is believed to be phosphorylated at Serine 54 during S phase and functions as a chromatin-bound signal that prevents reformation of prereplication complexes (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Cdc6 Antibody Phospho (pS54) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Williams, Shohet, Stillman: "A human protein related to yeast Cdc6p." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 1, pp. 142-7, 1997 (PubMed).

Hall, Kow, Nevis et al.: "Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage." in: Molecular biology of the cell, Vol. 18, Issue 9, pp. 3340-50, 2007 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN649522
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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