Cyclin-Dependent Kinase 2 (CDK2) (pTyr15) antibody

Antigen: Cyclin-Dependent Kinase 2 (CDK2)

Synonyms: p33(CDK2), A630093N05Rik, MGC849, 1810047K05Rik, 2810452K22Rik, RGD1563395, CINP, 2810452K22RIK, MGC84068, cinp, MGC166364, zgc:56598, zgc:77684, wu:fa10c02, CDK2

Binding Site: pTyr15

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649525

Additional Information

Alternative name: CDK2

Gene ID: 1017

UniProt: P24941

Description: Other names: CDK2, Cell division protein kinase 2, p33 protein kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding tyrosine 15 of human Cdk2 was used as immunogen. The antibody detects Cdk2 phosphorylated on tyrosine 15.

Molecular Weight: 33 kDA

Comments:

Background: Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kDa nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~20000 IP: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Cdk2 Antibody Phospho (pY15) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Elledge, Richman, Hall et al.: "CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 7, pp. 2907-11, 1992 (PubMed).

Gu, Rosenblatt, Morgan: "Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15." in: The EMBO journal, Vol. 11, Issue 11, pp. 3995-4005, 1992 (PubMed).

Morgan: "Principles of CDK regulation." in: Nature, Vol. 374, Issue 6518, pp. 131-4, 1995 (PubMed).

Fesquet, Labbé, Derancourt et al.: "The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues." in: The EMBO journal, Vol. 12, Issue 8, pp. 3111-21, 1993 (PubMed).