anti-CDK2 antibody (Cyclin-Dependent Kinase 2) (pTyr15)

Details for Product anti-CDK2 Antibody No. ABIN649525
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Antigen
Epitope
pTyr15
(55), (44), (21), (12), (10), (7), (7), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(250), (133), (114), (5), (5), (5), (4), (4), (3), (3), (2), (2)
Host
Rabbit
(233), (42), (1)
Clonality
Monoclonal
Conjugate
This CDK2 antibody is un-conjugated
(12), (8), (7), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1)
Application
Western Blotting (WB)
(236), (107), (70), (60), (42), (31), (30), (29), (14), (13), (12), (9), (8), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
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Request Want additional data for this product?

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Specificity A synthetic phospho-peptide corresponding to residues surrounding tyrosine 15 of human Cdk2 was used as immunogen. The antibody detects Cdk2 phosphorylated on tyrosine 15.
Alternative Name CDK2 (CDK2 Antibody Abstract)
Background Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kDa nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4).
Synonyms: CDK2, Cell division protein kinase 2, p33 protein kinase
Molecular Weight 33 kDA
Gene ID 1017
UniProt P24941
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Cycle, Chromatin, Cell Structure
Pathways PI3K-Akt Signaling Pathway
Application Notes The suggested dilution is: WB: = 1:5000-20000
Comment

Background: Cyclin-dependent protein kinase 2 (Cdk2), also known as cell division protein kinase 2, is a 34-kDa nuclear protein that regulates events within G1 and S phase in the human cell cycle (1-4). Cdk2 is inactive until a complex is formed with cyclin A, D, or E (2). Inhibitory phosphorylation occurs on Thr14 and Tyr15. Activation of the Cdk2 complex requires dephosphorylation of Thr14 and Tyr15 by cdc25 phosphatase and phosphorylation of Thr160, which is mediated by CAK (3,4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Cdk2 Antibody Phospho (pY15) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Fesquet, Labbé, Derancourt et al.: "The MO15 gene encodes the catalytic subunit of a protein kinase that activates cdc2 and other cyclin-dependent kinases (CDKs) through phosphorylation of Thr161 and its homologues." in: The EMBO journal, Vol. 12, Issue 8, pp. 3111-21, 1993 (PubMed).

Morgan: "Principles of CDK regulation." in: Nature, Vol. 374, Issue 6518, pp. 131-4, 1995 (PubMed).

Gu, Rosenblatt, Morgan: "Cell cycle regulation of CDK2 activity by phosphorylation of Thr160 and Tyr15." in: The EMBO journal, Vol. 11, Issue 11, pp. 3995-4005, 1992 (PubMed).

Elledge, Richman, Hall et al.: "CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 7, pp. 2907-11, 1992 (PubMed).

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Catalog No. ABIN649525
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