Checkpoint Kinase 1 (CHEK1) (C-Term) antibody

Antigen: Checkpoint Kinase 1 (CHEK1)

Synonyms: CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649535

Additional Information

Alternative name: Chk1

Gene ID: 1111

UniProt: O14757

Description: Other names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.

Molecular Weight: 56 kDA

Comments:

Background: Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10 FC: ~~ 1:20 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Chk1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Zeng, Forbes, Wu et al.: "Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1." in: Nature, Vol. 395, Issue 6701, pp. 507-10, 1998 (PubMed).

Martinho, Lindsay, Flaggs et al.: "Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses." in: The EMBO journal, Vol. 17, Issue 24, pp. 7239-49, 1999 (PubMed).

Zhao, Piwnica-Worms: "ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1." in: Molecular and cellular biology, Vol. 21, Issue 13, pp. 4129-39, 2001 (PubMed).