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Checkpoint Kinase 1 (CHEK1) (C-Term) antibody

Details for Product No. ABIN649535
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Antigen
Synonyms An08g10320, AO090003000441, CHK1, chk1, C85740, Chk1, rad27, id:ibd2720, zgc:56093, CHEK1
Epitope
C-Term
(47), (42), (31), (31), (20), (14), (11), (9), (9), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(384), (130), (123), (36), (26), (25), (14), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(338), (39), (12), (7)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(302), (158), (96), (56), (50), (47), (23), (19), (11), (4), (1), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN649535
450.00 $
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Specificity A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.
Alternative Name Chk1
Background Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).
Synonyms: CHEK1, CHK1, Serine/threonine-protein kinase Chk1
Molecular Weight 56 kDA
Gene ID 1111
UniProt O14757
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes IHC: = 1:250-500, WB: = 1:1000-10000
Comment

Background: Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Chk1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Zeng, Forbes, Wu et al.: "Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1." in: Nature, Vol. 395, Issue 6701, pp. 507-10, 1998 (PubMed).

Martinho, Lindsay, Flaggs et al.: "Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses." in: The EMBO journal, Vol. 17, Issue 24, pp. 7239-49, 1999 (PubMed).

Zhao, Piwnica-Worms: "ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1." in: Molecular and cellular biology, Vol. 21, Issue 13, pp. 4129-39, 2001 (PubMed).

Validation Images
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