|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Checkpoint Kinase 1 (CHEK1) (C-Term) antibody
|Synonyms||CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
|4 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to C-terminal residues of human Chk1 was used as an immunogen.|
|Molecular Weight||56 kDA|
Background: Chk1 is involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can bind to, phosphorylate (at serine 216) and inactivate cdc25c (3,4). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10 FC: ~~ 1:20 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Chk1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).
Zeng, Forbes, Wu et al.: "Replication checkpoint requires phosphorylation of the phosphatase Cdc25 by Cds1 or Chk1." in: Nature, Vol. 395, Issue 6701, pp. 507-10, 1998 (PubMed).
Martinho, Lindsay, Flaggs et al.: "Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses." in: The EMBO journal, Vol. 17, Issue 24, pp. 7239-49, 1999 (PubMed).
Zhao, Piwnica-Worms: "ATR-mediated checkpoint pathways regulate phosphorylation and activation of human Chk1." in: Molecular and cellular biology, Vol. 21, Issue 13, pp. 4129-39, 2001 (PubMed).