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Checkpoint Kinase 1 (CHEK1) (pSer280) antibody

Details for Product No. ABIN649537
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Antigen
Synonyms An08g10320, AO090003000441, CHK1, chk1, C85740, Chk1, rad27, id:ibd2720, zgc:56093, CHEK1
Epitope
pSer280
(46), (42), (31), (31), (20), (14), (11), (9), (9), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(384), (130), (123), (36), (26), (25), (14), (4), (3), (2), (2), (2), (1)
Host
Rabbit
(338), (39), (12), (7)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (9), (9), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4)
Application
Western Blotting (WB)
(302), (158), (97), (56), (50), (47), (23), (19), (11), (4), (1), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN649537
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Specificity A phospho-specific peptide corresponding to residues surrounding Serine 280 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated on Serine 280.
Alternative Name CHK1
Background Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes. Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1). It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4). Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity. Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5).
Synonyms: CHEK1, CHK1, Serine/threonine-protein kinase Chk1
Molecular Weight 56 kDA
Gene ID 1111
UniProt O14757
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure
Application Notes The suggested dilution is: WB: = 1:2500-5000
Comment

Background: Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes. Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1). It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4). Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity. Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Chk1 Antibody Phospho (pS280) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Liu, Guntuku, Cui et al.: "Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint." in: Genes & development, Vol. 14, Issue 12, pp. 1448-59, 2000 (PubMed).

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