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Checkpoint Kinase 1 (CHEK1) (pSer280) antibody

Antigen

Checkpoint Kinase 1 (CHEK1)
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1
Binding Site
Alternatives

pSer280
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649537
Quantity 100ul  (Variants)
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CHK1
Gene ID 1111
UniProt O14757
Description Other names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1
Characteristics Rabbit Monoclonal Antibody
Specificity A phospho-specific peptide corresponding to residues surrounding Serine 280 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated on Serine 280.
Molecular Weight 56 kDA
Comments

Background: Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes. Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1). It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4). Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity. Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:2500~5000 IP: ~~ 1:50
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Chk1 Antibody Phospho (pS280) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure
Restrictions For Research Use only

Publications
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Product Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Liu, Guntuku, Cui et al.: "Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint." in: Genes & development, Vol. 14, Issue 12, pp. 1448-59, 2000 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts Rabbit (184), Mouse (20), Goat (6), Sheep (4)
Reactivities Human (202), Rat (Rattus) (91), Mouse (Murine) (83), Horse (Equine) (10), Cow (Bovine) (9), Chicken (8), Dog (Canine) (7), Pig (Porcine) (6), Monkey (3), Xenopus laevis (2), Yeast (Saccharomyces cerevisiae) (2), Zebrafish (2), Cat (Feline) (1), Rabbit (1), Yeast (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (138), ELISA (82), Immunofluorescence (IF) (61), Immunohistochemistry (IHC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunoprecipitation (IP) (14), Immunocytochemistry (ICC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Dot Blot (DB) (2), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (2)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (4), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes Ser296 (17), pSer317 (17), pSer280 (15), pSer345 (13), Internal Region (7), pSer296 (7), C-Term (5), Ser280 (4), N-Term (3), pSer296,Internal Region (3), AA 312-327 (2), Center (2), AA 1-474 (1), AA 250-300 (1), Middle Region (1), pSer286 (1), pSer301 (1), pThr14 (1), pThr29 (1)