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Checkpoint Kinase 1 (CHEK1) (pSer280) antibody
|Synonyms||CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1|
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A phospho-specific peptide corresponding to residues surrounding Serine 280 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated on Serine 280.|
|Molecular Weight||56 kDA|
Background: Chk1 is an evolutionarily conserved protein kinase that has been implicated in cell cycle checkpoint control in lower eukaryotes. Chk1 plays an essential role in the mammalian DNA damage checkpoint, embryonic development, and tumor suppression (1). It is activated in response to replication blocks and genotoxic stress, via phosphorylation of Serine 317 and Serine 345 (2). Activated Chk1 can bind to, phosphorylate (at Serine 216) and inactivate cdc25c (3, 4). Chk1 is phosphorylated by ATR in response to ultraviolet irradiation and inhibition of DNA replication, and by ATM in response to ionizing irradiation, resulting in enhanced kinase activity. Chk1 is phosphorylated at Serine 280 by AKT1/PKB, which is thought to promote mono and/or diubiquitination (5).
|Application Notes||The suggested dilution is: WB: ~~ 1:2500~5000 IP: ~~ 1:50|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Chk1 Antibody Phospho (pS280) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).
Liu, Guntuku, Cui et al.: "Chk1 is an essential kinase that is regulated by Atr and required for the G(2)/M DNA damage checkpoint." in: Genes & development, Vol. 14, Issue 12, pp. 1448-59, 2000 (PubMed).