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Checkpoint Kinase 1 (CHEK1) (pSer296) antibody

Antigen

Checkpoint Kinase 1 (CHEK1)
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1
Binding Site
Alternatives

pSer296
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649538
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CHK1
Gene ID 1111
UniProt O14757
Description Other names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1
Characteristics Rabbit Monoclonal Antibody
Specificity A phospho specific peptide corresponding to residues surrounding Serine 296 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated at Serine 296.
Molecular Weight 56 kDA
Comments

Background: Chk1 is a serine/threonine-protein kinase involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can inactivate cdc25c through phosphorylation at Serine 216 (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~2000 ICC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Chk1 Antibody Phospho (pS296) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).

Martinho, Lindsay, Flaggs et al.: "Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses." in: The EMBO journal, Vol. 17, Issue 24, pp. 7239-49, 1999 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts Rabbit (173), Mouse (20), Goat (6), Sheep (4)
Reactivities Human (192), Rat (Rattus) (82), Mouse (Murine) (71), Horse (Equine) (10), Cow (Bovine) (9), Chicken (8), Dog (Canine) (7), Pig (Porcine) (6), Monkey (2), Xenopus laevis (2), Yeast (Saccharomyces cerevisiae) (2), Zebrafish (2), Cat (Feline) (1), Rabbit (1), Yeast (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (129), ELISA (81), Immunofluorescence (IF) (60), Immunohistochemistry (IHC) (43), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (32), Immunoprecipitation (IP) (15), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (4), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (2)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (4), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes pSer317 (23), pSer280 (20), Ser296 (17), pSer345 (16), Internal Region (7), C-Term (6), pSer296 (6), Ser280 (4), AA 312-327 (3), N-Term (3), Center (2), AA 1-474 (1), AA 250-300 (1), AA 309-321 (1), Middle Region (1), pSer286 (1), pSer301 (1), pThr14 (1), pThr29 (1)