|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
|Synonyms||CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1|
Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: CHEK1, CHK1, Serine/threonine-protein kinase Chk1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A phospho specific peptide corresponding to residues surrounding Serine 296 of human Chk1 was used as an immunogen. The antibody only detects Chk1 phosphorylated at Serine 296.|
|Molecular Weight||56 kDA|
Background: Chk1 is a serine/threonine-protein kinase involved in cell cycle arrest when DNA damage has occurred, and it plays an important role in DNA damage checkpoint, embryonic development and tumor suppression (1). Chk1 is activated in response to replication blocks and genotoxic stress, via phosphorylation of serines 317 and 345 (2). Activated Chk1 can inactivate cdc25c through phosphorylation at Serine 216 (3,4 ). Phosphorylation of cdc25c prevents activation of the CDC2-cyclin B complex and prevents entry into M-phase (3, 4).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~2000 ICC: ~~ 1:100~250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Chk1 Antibody Phospho (pS296) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Signaling, Cell Structure|
|Restrictions||For Research Use only|
Sanchez, Wong, Thoma et al.: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997 (PubMed).
Martinho, Lindsay, Flaggs et al.: "Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses." in: The EMBO journal, Vol. 17, Issue 24, pp. 7239-49, 1999 (PubMed).