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Jun Proto-Oncogene (JUN) (C-Term) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Binding Site
Alternatives

C-Term
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN649544
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name c-Jun
Gene ID 3725
UniProt P05412
Description Other names: JUN, Transcription factor AP-1, Proto-oncogene c-jun,V-jun avian sarcoma virus 17 oncogene homolog,p39
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to the C-terminal residues of human c-Jun was used as immunogen.
Molecular Weight 43 kDA
Comments

Background: c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:50 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage c-Jun Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Smeal, Binetruy, Mercola et al.: "Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73." in: Nature, Vol. 354, Issue 6353, pp. 494-6, 1992 (PubMed).

Bohmann, Bos, Admon et al.: "Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1." in: Science (New York, N.Y.), Vol. 238, Issue 4832, pp. 1386-92, 1988 (PubMed).

Dérijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).

Kyriakis, Banerjee, Nikolakaki et al.: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (351), Mouse (9), Chicken (1), Sheep (1)
Reactivities Human (355), Mouse (Murine) (238), Rat (Rattus) (225), Cow (Bovine) (86), Dog (Canine) (86), Pig (Porcine) (82), Chicken (77), Sheep (Ovine) (62), Horse (Equine) (25), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (257), Immunohistochemistry (IHC) (124), ELISA (115), Immunofluorescence (IF) (101), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunoprecipitation (IP) (40), Immunocytochemistry (ICC) (12), Dot Blot (Dot) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (DB) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy5.5 (1)
Epitopes pSer73 (20), pThr93,pThr91 (17), pSer63 (16), pThr239 (16), pThr91 (15), pTyr170 (15), pSer243 (14), Internal Region (12), pThr93 (10), N-Term (7), pThr249 (7), C-Term (6), Ser73 (5), Thr239 (4), pThr91,pThr93 (4), Ser243 (3), Ser63 (3), Tyr170 (3), AA 266-285 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr243 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,Glu248 (1), Middle Region (1), Ser73,Phosphospecific (1), pSer213 (1), pSer539,pSer535 (1), pThr18 (1), pThr231 (1), pTyr981,pTyr980 (1)