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Jun Proto-Oncogene (JUN) (pSer63) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Binding Site
Alternatives

pSer63
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN649546
Quantity 100ul  (Variants)
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name c-Jun
Gene ID 3725
UniProt P05412
Description Other names: JUN, Transcription factor AP-1, Proto-oncogene c-jun,V-jun avian sarcoma virus 17 oncogene homolog,p39
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 63 of human c-Jun was used as immunogen. The antibody only detects c-Jun phosphorylated on Serine 63. Predicted to cross-react with bovine, based on sequence homology.
Molecular Weight 48 kDA
Comments

Background: c-Jun is a component of the transcription factor AP-1 (1). The transcriptional activity of c-Jun is regulated by phosphorylation at serines 63 and 73 (1,2). Extracellular signals, including growth factors, transforming oncoproteins and UV light, stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun-dependent transcription (3,4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:500 IP: ~~ 1:80 FC: ~~ 1:100 ICC: ~~ 1:100~200 IHC: ~~ 1:50~100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage c-Jun Antibody Phospho (pS63) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Restrictions For Research Use only

Publications
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Product Smeal, Binetruy, Mercola et al.: "Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73." in: Nature, Vol. 354, Issue 6353, pp. 494-6, 1992 (PubMed).

Bohmann, Bos, Admon et al.: "Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1." in: Science (New York, N.Y.), Vol. 238, Issue 4832, pp. 1386-92, 1988 (PubMed).

Dérijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).

Kyriakis, Banerjee, Nikolakaki et al.: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (348), Mouse (9), Chicken (1), Goat (1), Sheep (1)
Reactivities Human (350), Rat (Rattus) (197), Mouse (Murine) (190), Cow (Bovine) (87), Dog (Canine) (86), Pig (Porcine) (82), Chicken (77), Sheep (Ovine) (62), Horse (Equine) (25), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (253), Immunohistochemistry (IHC) (124), ELISA (118), Immunofluorescence (IF) (101), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunoprecipitation (IP) (41), Dot Blot (Dot) (6), Immunocytochemistry (ICC) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy5.5 (1)
Epitopes pSer73 (26), pSer63 (19), pThr239 (19), pTyr170 (18), pThr91 (17), pThr93,pThr91 (17), pSer243 (16), Internal Region (12), pThr93 (12), C-Term (8), pThr249 (8), N-Term (7), Ser73 (5), Thr239 (4), pThr91,pThr93 (4), Ser243 (3), Ser63 (3), Tyr170 (3), pThr243 (3), AA 266-285 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,Glu248 (1), Middle Region (1), Ser73,Phosphospecific (1), Sumoylation Site (1), non-pSer73 (1), pSer213 (1), pSer539,pSer535 (1), pThr18 (1), pThr231 (1), pTyr981,pTyr980 (1)