anti-C-JUN antibody (Jun Proto-Oncogene) (pThr91)

Details for Product anti-JUN Antibody No. ABIN649548
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Antigen
Synonyms
BSK, BSK/DJNK, Bsk, CG5680, D-JNK, D-junk, DBSK/JNK, DJNK, DJNK/bsk, Dmel\\CG5680, JNK, JNK/SAPK, Jnk, Junk, SAPKa, c-Jun, dJNK, jnk, Jun, AP-1, JUN, B230310J22Rik, p39, Junc, c-jun, AP1, fj36h07, wu: ... show more
BSK, BSK/DJNK, Bsk, CG5680, D-JNK, D-junk, DBSK/JNK, DJNK, DJNK/bsk, Dmel\\CG5680, JNK, JNK/SAPK, Jnk, Junk, SAPKa, c-Jun, dJNK, jnk, Jun, AP-1, JUN, B230310J22Rik, p39, Junc, c-jun, AP1, fj36h07, wu:fj36h07, zgc:65863, CG2275, D-Jun, D-jun, D-jun/Jra, DJUN, DJun, Djun, Dmel\\CG2275, JRA, V, cJun, d-JRA, d-Jun, d-jun, dAP-1, dJRA, dJUN, dJra, dJun, djun, dm-Jun, jra, jun, l(2)46Ef, l(2)IA109, l(2R)IA109 show less
Epitope
pThr91
(56), (47), (43), (41), (40), (39), (37), (29), (22), (17), (15), (14), (12), (12), (12), (11), (10), (9), (8), (7), (7), (7), (7), (7), (7), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(724), (425), (377), (13), (12), (12), (10), (9), (9), (8), (5), (5), (5), (5), (3), (3), (2), (2), (2), (1)
Host
Rabbit
(696), (49), (4), (2)
Clonality
Monoclonal
Conjugate
This C-JUN antibody is un-conjugated
(18), (18), (18), (10), (10), (10), (8), (8), (8), (8), (8), (8), (8), (8)
Application
Western Blotting (WB)
(618), (363), (202), (104), (95), (79), (73), (66), (18), (17), (16), (7), (6), (5), (3), (2), (1), (1)
Pubmed 4 references available
Quantity 100 μL
Options
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Specificity A synthetic peptide corresponding to residues surrounding Threonine 91 of human c-Jun was used as an immunogen. The antibody only recognizes c-Jun phosphorylated at Threonine 91.
Alternative Name c-Jun (JUN Antibody Abstract)
Background C-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).
Synonyms: JUN, Transcription factor AP-1, Proto-oncogene c-jun,V-jun avian sarcoma virus 17 oncogene homolog,p39
Molecular Weight 48 kDA
Gene ID 3725
UniProt P05412
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-2000
Comment

Background: c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment c-Jun Antibody Phospho (pT91) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Weiss, Schneider, Wagner et al.: "JNK phosphorylation relieves HDAC3-dependent suppression of the transcriptional activity of c-Jun." in: The EMBO journal, Vol. 22, Issue 14, pp. 3686-95, 2003 (PubMed).

Eferl, Ricci, Kenner et al.: "Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53." in: Cell, Vol. 112, Issue 2, pp. 181-92, 2003 (PubMed).

Behrens, Sibilia, Wagner: "Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation." in: Nature genetics, Vol. 21, Issue 3, pp. 326-9, 1999 (PubMed).

Papavassiliou, Treier, Bohmann: "Intramolecular signal transduction in c-Jun." in: The EMBO journal, Vol. 14, Issue 9, pp. 2014-9, 1995 (PubMed).

Validation Images
Catalog No. ABIN649548
421.43 $
Plus shipping costs $45.00
Quantity
Price
100 μL
421.43 $

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