Jun Proto-Oncogene (JUN) (pThr91) antibody

Antigen: Jun Proto-Oncogene (JUN)

Synonyms: AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun

Binding Site: pThr91

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649548

Additional Information

Alternative name: c-Jun

Gene ID: 3725

UniProt: P05412

Description: Other names: JUN, Transcription factor AP-1, Proto-oncogene c-jun,V-jun avian sarcoma virus 17 oncogene homolog,p39

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues surrounding Threonine 91 of human c-Jun was used as an immunogen. The antibody only recognizes c-Jun phosphorylated at Threonine 91.

Molecular Weight: 48 kDA

Comments:

Background: c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:20

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: c-Jun Antibody Phospho (pT91) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Publications

Product: Papavassiliou, Treier, Bohmann: "Intramolecular signal transduction in c-Jun." in: The EMBO journal, Vol. 14, Issue 9, pp. 2014-9, 1995 (PubMed).

Behrens, Sibilia, Wagner: "Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation." in: Nature genetics, Vol. 21, Issue 3, pp. 326-9, 1999 (PubMed).

Eferl, Ricci, Kenner et al.: "Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53." in: Cell, Vol. 112, Issue 2, pp. 181-92, 2003 (PubMed).

Weiss, Schneider, Wagner et al.: "JNK phosphorylation relieves HDAC3-dependent suppression of the transcriptional activity of c-Jun." in: The EMBO journal, Vol. 22, Issue 14, pp. 3686-95, 2003 (PubMed).