Jun Proto-Oncogene (JUN) (pThr93) antibody

Details for Product No. ABIN649549
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Antigen
Synonyms p39, B230310J22Rik, AP1, AP-1, c-Jun, Junc, c-jun, Jun, JUN, fj36h07, zgc:65863, wu:fj36h07
Epitope
pThr93
(70), (60), (55), (46), (37), (37), (29), (19), (13), (10), (9), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(598), (419), (387), (154), (152), (150), (139), (10), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Host
Rabbit
(555), (57), (2), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Application
Western Blotting (WB)
(466), (222), (180), (127), (119), (66), (49), (21), (19), (8), (8), (6), (2), (1), (1), (1)
Pubmed 4 references available
Catalog no. ABIN649549
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues surrounding Threonine 93 of human c-Jun was used as an immunogen. The antibody only detects c-Jun phosphorylated at Threonine 93.
Alternative Name c-Jun
Background C-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).
Synonyms: JUN, Transcription factor AP-1, Proto-oncogene c-jun,V-jun avian sarcoma virus 17 oncogene homolog,p39
Molecular Weight 48 kDA
Gene ID 3725
UniProt P05412
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-5000
Comment

Background: c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment c-Jun Antibody Phospho (pT93) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Papavassiliou, Treier, Bohmann: "Intramolecular signal transduction in c-Jun." in: The EMBO journal, Vol. 14, Issue 9, pp. 2014-9, 1995 (PubMed).

Behrens, Sibilia, Wagner: "Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation." in: Nature genetics, Vol. 21, Issue 3, pp. 326-9, 1999 (PubMed).

Eferl, Ricci, Kenner et al.: "Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53." in: Cell, Vol. 112, Issue 2, pp. 181-92, 2003 (PubMed).

Weiss, Schneider, Wagner et al.: "JNK phosphorylation relieves HDAC3-dependent suppression of the transcriptional activity of c-Jun." in: The EMBO journal, Vol. 22, Issue 14, pp. 3686-95, 2003 (PubMed).

Hosts (555), (57), (2), (1), (1)
Reactivities (598), (419), (387), (154), (152), (150), (139), (10), (7), (6), (3), (3), (3), (3), (3), (2), (2), (2), (2)
Applications (466), (222), (180), (127), (119), (66), (49), (21), (19), (8), (8), (6), (2), (1), (1), (1)
Conjugates (12), (12), (12), (12), (12), (12), (12), (12), (12), (12), (12)
Epitopes (70), (60), (55), (46), (37), (37), (29), (19), (13), (10), (9), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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