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Jun Proto-Oncogene (JUN) (pThr93) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Binding Site
Alternatives

pThr93
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunoprecipitation (IP)
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN649549
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name c-Jun
Gene ID 3725
UniProt P05412
Description Other names: JUN, Transcription factor AP-1, Proto-oncogene c-jun,V-jun avian sarcoma virus 17 oncogene homolog,p39
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues surrounding Threonine 93 of human c-Jun was used as an immunogen. The antibody only detects c-Jun phosphorylated at Threonine 93.
Molecular Weight 48 kDA
Comments

Background: c-Jun is a major component of the heterodimeric transcription factor AP-1 and is essential for embryonic development (1). It mediates several cellular processes, including proliferation and survival, and is upregulated in many carcinomas (2). Transactivation of c-Jun is regulated by Jun-N-terminal Kinases (JNKs) through phosphorylation at Serine 63 and 73, as well as at threonine 91 and 93 (3). Evidence supports that phosphorylation in NH2-terminal residues of c-Jun stimulates the dephosphorylation of the COOH-terminal sites, and consequently increases the DNA-binding activity of the transcription factor (4). c-Jun N-terminal kinase (JNK) isoforms are required for the phosphorylation of Threonine 91 and 93 in response to anisomycin in macrophages and TNF-alpha or anisomycin in fibroblasts (5).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:50
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage c-Jun Antibody Phospho (pT93) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Papavassiliou, Treier, Bohmann: "Intramolecular signal transduction in c-Jun." in: The EMBO journal, Vol. 14, Issue 9, pp. 2014-9, 1995 (PubMed).

Behrens, Sibilia, Wagner: "Amino-terminal phosphorylation of c-Jun regulates stress-induced apoptosis and cellular proliferation." in: Nature genetics, Vol. 21, Issue 3, pp. 326-9, 1999 (PubMed).

Eferl, Ricci, Kenner et al.: "Liver tumor development. c-Jun antagonizes the proapoptotic activity of p53." in: Cell, Vol. 112, Issue 2, pp. 181-92, 2003 (PubMed).

Weiss, Schneider, Wagner et al.: "JNK phosphorylation relieves HDAC3-dependent suppression of the transcriptional activity of c-Jun." in: The EMBO journal, Vol. 22, Issue 14, pp. 3686-95, 2003 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (351), Mouse (9), Chicken (1), Sheep (1)
Reactivities Human (355), Mouse (Murine) (238), Rat (Rattus) (225), Cow (Bovine) (86), Dog (Canine) (86), Pig (Porcine) (82), Chicken (77), Sheep (Ovine) (62), Horse (Equine) (25), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (257), Immunohistochemistry (IHC) (125), ELISA (115), Immunofluorescence (IF) (101), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunoprecipitation (IP) (40), Immunocytochemistry (ICC) (13), Dot Blot (Dot) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (DB) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy5.5 (1)
Epitopes pSer73 (20), pThr93,pThr91 (17), pSer63 (16), pThr239 (16), pThr91 (15), pTyr170 (15), pSer243 (14), Internal Region (12), pThr93 (9), C-Term (7), N-Term (7), pThr249 (7), Ser73 (5), Thr239 (4), pThr91,pThr93 (4), Ser243 (3), Ser63 (3), Tyr170 (3), AA 266-285 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr243 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,Glu248 (1), Middle Region (1), Ser73,Phosphospecific (1), pSer213 (1), pSer539,pSer535 (1), pThr18 (1), pThr231 (1), pTyr981,pTyr980 (1)