V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog (KIT) (C-Term) antibody

Antigen: V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog (KIT)

Synonyms: PBT, SCFR, C-Kit, CD117, W, Bs, Fdc, Ssm, SCO1, SCO5, SOW3, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3, c-kit, kit-A, XKrk1, KIT, MGF, LOC100221238, C-KIT

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649550

Additional Information

Alternative name: c-Kit/CD117

Gene ID: 3815

UniProt: P10721

Description: Other names: KIT, Mast/stem cell growth factor receptor, Proto-oncogene tyrosine-protein kinase Kit

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the C-terminus (cytoplasmic domain) of human c-Kit was used as an immunogen. The antibody does not cross-react with other CSF-1/PDGF receptor family member.

Molecular Weight: 145 kDA

Comments:

Background: Member of the Tyrosine Kinase Receptor (TKRs) and highly homologous to receptor PDF and CSF-1 (1-2), c-Kit is a cellular homologue of the oncogene v-Kit of the Hardy Zuckerman 4-feline sarcoma virus (3). Activation of c-Kit tyrosine kinase by SCF (Stem Cell factor) leads to autophosphorylation and association of c-Kit with substrate PI3K (4). Missense substitution within the cellular tyrosine kinase may cause Piebaldism an autosomal dominant disorder of the skin (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: c-Kit/CD117 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Spritz, Giebel, Holmes: "Dominant negative and loss of function mutations of the c-kit (mast/stem cell growth factor receptor) proto-oncogene in human piebaldism." in: American journal of human genetics, Vol. 50, Issue 2, pp. 261-9, 1992 (PubMed).

Yarden, Kuang, Yang-Feng et al.: "Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand." in: The EMBO journal, Vol. 6, Issue 11, pp. 3341-51, 1988 (PubMed).

Qiu, Ray, Brown et al.: "Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family--oncogenic activation of v-kit involves deletion of extracellular domain and C terminus." in: The EMBO journal, Vol. 7, Issue 4, pp. 1003-11, 1988 (PubMed).

Besmer, Murphy, George et al.: "A new acute transforming feline retrovirus and relationship of its oncogene v-kit with the protein kinase gene family." in: Nature, Vol. 320, Issue 6061, pp. 415-21, 1986 (PubMed).