V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (pThr58), (pSer62) antibody

Details for Product No. ABIN649555
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Antigen
Synonyms LOC100136746, RNCMYC, c-myc, mMyc, AU016757, Myc2, Niard, Nird, bHLHe39, MYC, cmyc, zc-myc, MRTL, MYCC, c-Myc, C-MYC, CMYC, CMyc
Epitope
pThr58, pSer62
(51), (42), (41), (32), (24), (19), (15), (14), (12), (12), (11), (7), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(468), (198), (170), (31), (27), (25), (24), (16), (4), (3), (3), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(347), (147), (19), (14), (3), (3)
Clonality
Monoclonal
Conjugate
Un-conjugated
(31), (28), (22), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(373), (177), (143), (97), (93), (85), (51), (50), (40), (20), (17), (10), (8), (6), (2), (1)
Pubmed 2 references available
Quantity 100 µL
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Catalog No. ABIN649555
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Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology.
Alternative Name C-Myc
Background Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).
Synonyms: MYC, Myc proto-oncogene protein, c-Myc,Transcription factor p64
Molecular Weight 57-70 kDA
Gene ID 4609
UniProt P01106
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure
Application Notes WB: = 1:1000
Comment

Background: Oncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment c-Myc Antibody Phospho (pT58/pS62) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Nau, Brooks, Battey et al.: "L-myc, a new myc-related gene amplified and expressed in human small cell lung cancer." in: Nature, Vol. 318, Issue 6041, pp. 69-73, 1985 (PubMed).

Gupta, Seth, Davis: "Transactivation of gene expression by Myc is inhibited by mutation at the phosphorylation sites Thr-58 and Ser-62." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3216-20, 1993 (PubMed).

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