C-reactive (C-Term) antibody

Antigen: C-reactive

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649562

Additional Information

Gene ID: 1401

UniProt: P02741

Description: Other names: CRP, PTX1, C-reactive protein(1-205)

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the C-term of human c-Reactive protein was used as immunogen. This antibody is predicted to detect the splice isoform 2.

Molecular Weight: 23/117.5 kDA

Comments:

Background: Human c-Reactive protein (CRP) was discovered in serum of patients as a nonantibody precipitin of the C-polysaccharide from pneumococci(1). During acute infection, the amounts of circulating CPR increases 100-1000 fold (2). Serum CRP values are routinely measured to detect or monitor many human diseases and minor elevations of C-reactive protein are predictive of cardiovascular events in patients with coronary heart disease (3). CRP promotes host defense, is an efficient activator of the classical C cascade, and shown to be opsonic (4).The opsonic and C activation activities are responsible for the nonspecific host defense activities providing protection in the preimmune stage of acute infections. Additionally, IL-1 and IL-6 are known to stimulate CRP biosyntesis (5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:50 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: C-reactive Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Mortensen, Osmand, Lint et al.: "Interaction of C-reactive protein with lymphocytes and monocytes: complement-dependent adherence and phagocytosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 117, Issue 3, pp. 774-81, 1976 (PubMed).

Kushner: "The phenomenon of the acute phase response." in: Annals of the New York Academy of Sciences, Vol. 389, pp. 39-48, 1982 (PubMed).

Kleemann, Gervois, Verschuren et al.: "Fibrates down-regulate IL-1-stimulated C-reactive protein gene expression in hepatocytes by reducing nuclear p50-NFkappa B-C/EBP-beta complex formation." in: Blood, Vol. 101, Issue 2, pp. 545-51, 2003 (PubMed).

Ridker, Rifai, Rose et al.: "Comparison of C-reactive protein and low-density lipoprotein cholesterol levels in the prediction of first cardiovascular events." in: The New England journal of medicine, Vol. 347, Issue 20, pp. 1557-65, 2002 (PubMed).

Ohara, Granato, Moallem et al.: "Dopaminergic input to GABAergic neurons in the rostral agranular insular cortex of the rat." in: Journal of neurocytology, Vol. 32, Issue 2, pp. 131-41, 2004 (PubMed).