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Details for Product No. ABIN649566

CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody

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Antigen
Synonyms CREB, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, creb, creb1, zgc:55598, CREB1, Creb1
Epitope
»Alternatives pSer133
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 3 references available
Catalog no. ABIN649566
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB was used as immunogen. The antibody only detects CREB phosphorylated on Serine 133. Predicted to cross-react with bovine, chicken, and zebra fish, based on sequence homology.
Alternative Name Creb
Background CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).
Synonyms: CREB1, Cyclic AMP-responsive element-binding protein 1
Molecular Weight 40 kDA
Gene ID 1385
UniProt P16220
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:500~2000 IP: ~~ 1:40 ICC: ~~ 1:250 IHC: ~~ 1:250
Comment

Background: CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Creb Antibody Phospho (pS133) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Sheng, Thompson, Greenberg: "CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases." in: Science (New York, N.Y.), Vol. 252, Issue 5011, pp. 1427-30, 1991 (PubMed).

Xing, Ginty, Greenberg: "Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase." in: Science (New York, N.Y.), Vol. 273, Issue 5277, pp. 959-63, 1996 (PubMed).

Xing, Kornhauser, Xia et al.: "Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 1946-55, 1998 (PubMed).

Alternatives for antigen "CAMP Responsive Element Binding Protein 1 (CREB1)", type "Antibodies"
Hosts (284), (58), (1)
Reactivities (323), (227), (215), (75), (69), (68), (45), (8), (6), (6), (5), (1), (1), (1)
Applications (249), (129), (110), (84), (60), (36), (31), (19), (9), (3), (2), (2), (2), (2), (2), (1), (1)
Conjugates (9), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (74), (35), (24), (20), (14), (9), (7), (5), (5), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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