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CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody

Antigen

CAMP Responsive Element Binding Protein 1 (CREB1)
Synonyms CREB, MGC9284, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, JAL, hJAL, ATCE1, CREB3, CREB4, AIBZIP, CREB1, creb1, MGC52669, creb, MGC69285, MGC55598, zgc:55598, Creb1, DKFZp468J2311
Binding Site
Alternatives

pSer133
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649566
Quantity 100ul  (Variants)
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Creb
Gene ID 1385
UniProt P16220
Description Other names: CREB1, Cyclic AMP-responsive element-binding protein 1
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB was used as immunogen. The antibody only detects CREB phosphorylated on Serine 133. Predicted to cross-react with bovine, chicken, and zebra fish, based on sequence homology.
Molecular Weight 40 kDA
Comments

Background: CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:500~2000 IP: ~~ 1:40 ICC: ~~ 1:250 IHC: ~~ 1:250
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Creb Antibody Phospho (pS133) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
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Product Sheng, Thompson, Greenberg: "CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases." in: Science (New York, N.Y.), Vol. 252, Issue 5011, pp. 1427-30, 1991 (PubMed).

Xing, Ginty, Greenberg: "Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase." in: Science (New York, N.Y.), Vol. 273, Issue 5277, pp. 959-63, 1996 (PubMed).

Xing, Kornhauser, Xia et al.: "Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 1946-55, 1998 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "CAMP Responsive Element Binding Protein 1 (CREB1)", type "Antibodies"
Hosts Rabbit (171), Mouse (50)
Reactivities Human (206), Rat (Rattus) (128), Mouse (Murine) (114), Cow (Bovine) (59), Pig (Porcine) (59), Sheep (Ovine) (56), Chicken (55), Dog (Canine) (46), Horse (Equine) (15), Monkey (4), Xenopus laevis (2), Zebrafish (2), Cat (Feline) (1)
Applications Western Blotting (WB) (139), ELISA (71), Immunofluorescence (IF) (69), Immunohistochemistry (IHC) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (41), Immunoprecipitation (IP) (19), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Flow Cytometry (FACS) (5), Immunocytochemistry (ICC) (4), Gel Shift (GS) (2), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (Dot) (1)
Conjugates Alexa Fluor 488 (7), Alexa Fluor 350 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), Biotin (5), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2)
Epitopes pSer133 (46), pSer129 (18), pSer142 (9), N-Term (7), Ser129 (5), pSer121 (5), Internal Region (4), Ser133 (4), Center (2), pS133 (2), phosphospecific (2), AA 1-96 (1), AA 100-150 (1), AA 122-147,pSer133 (1), AA 136-341 (1), AA 152-179 (1), AA 5-24 (1), AA 5-45 (1), Ser142 (1), Thr100 (1), pSer129, pSer133 (1), pThr100 (1), pThr244 (1), pTyr766 (1)