|+1 404 474 4654|
|+1 888 205 9894 (TF)|
CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
|Synonyms||CREB, MGC9284, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, JAL, hJAL, ATCE1, CREB3, CREB4, AIBZIP, CREB1, creb1, MGC52669, creb, MGC69285, MGC55598, zgc:55598, Creb1, DKFZp468J2311|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|3 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: CREB1, Cyclic AMP-responsive element-binding protein 1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser133 of human CREB was used as immunogen. The antibody only detects CREB phosphorylated on Serine 133. Predicted to cross-react with bovine, chicken, and zebra fish, based on sequence homology.|
|Molecular Weight||40 kDA|
Background: CREB is a transcription factor protein that binds the cAMP response element (CRE), thereby stimulating transcription of this sequence. CREB plays a role in propagating numerous extracellular signals to the nuclues (1-3). CREB is activated by phosphorylation at the Ser133 position by various signaling pathways, and some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV and MAPKAPK-2 (1-3).
|Application Notes||The suggested dilution is: WB: ~~ 1:500~2000 IP: ~~ 1:40 ICC: ~~ 1:250 IHC: ~~ 1:250|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Creb Antibody Phospho (pS133) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Sheng, Thompson, Greenberg: "CREB: a Ca(2+)-regulated transcription factor phosphorylated by calmodulin-dependent kinases." in: Science (New York, N.Y.), Vol. 252, Issue 5011, pp. 1427-30, 1991 (PubMed).
Xing, Ginty, Greenberg: "Coupling of the RAS-MAPK pathway to gene activation by RSK2, a growth factor-regulated CREB kinase." in: Science (New York, N.Y.), Vol. 273, Issue 5277, pp. 959-63, 1996 (PubMed).
Xing, Kornhauser, Xia et al.: "Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 1946-55, 1998 (PubMed).