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Details for Product No. ABIN649576

Cullin 1 (CUL1) antibody

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Antigen
Synonyms cullin-1, Cullin-1, ATCUL1, AUXIN RESISTANT 6, AXR6, T10P11.26, T10P11_26, cullin 1, LOC100282292, Cul1, zgc:55747, zgc:77500, wu:fc13a10, CUL1
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Pubmed 1 reference available
Catalog no. ABIN649576
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues on the C-terminus of human CUL1 was used as an immunogen.
Alternative Name CUL1
Background Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation (2). The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization (3).
Synonyms: CUL1, Cullin-1
Molecular Weight 90 kDA
Gene ID 8454
UniProt Q13616
Research Area Ubiquitin-related antibodies, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:2000~10000 IP: ~~ 1:10 ICC: ~~ 1:100~250 IHC: ~~ 1:100~250
Comment

Background: Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes (1). It has been shown that the SKP1, CUL1, F-box (SCF) containing pathway, although similarly used by the mammalian CUL1, is not shared by other cullin members. This implies that most cullins may use a SKP1/F-box-independent pathway to facilitate protein degradation (2). The majority of CUL1 has been found in a complex with CAND1 and ROC1, independent of SKP1 and F-box protein SKP2. Data suggests that CAND1 regulates the formation of the SCF complex, and its dissociation from CUL1 is coupled with the incorporation of F-box proteins into the SCF complex, causing their destabilization (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment CUL1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).

Alternatives for antigen "Cullin 1 (CUL1)", type "Antibodies"
Hosts (71), (5), (1)
Reactivities (74), (35), (18), (13), (12), (1), (1), (1), (1)
Applications (60), (29), (26), (21), (15), (10), (9), (2), (1), (1), (1), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (24), (3), (2), (2), (1), (1), (1), (1)
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