CUL4A/B antibody

Antigen: CUL4A/B

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649580

Additional Information

Gene ID: 8450

UniProt: Q13620

Description: Other names: KHDRBS1, SAM68, KH domain-containing, RNA-binding, signal transduction-associated protein 1, p68

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human CUL4B was used as an immunogen.

Molecular Weight: 88/102 kDA

Comments:

Background: Cullin proteins assemble a large number of RING E3 ubiquitin ligases and regulate various physiological processes, including regulating replication and transcription (1-2). Two cullin 4 (CUL4) proteins, CUL4A and CUL4B, have been identified. CUL4A and CUL4B are two closely related proteins, coded by two different genes. The amino acid sequences of CUL4A and CUL4B show 80% similarity, differing by the unique and extended N-terminus of CUL4B. CUL4B is reported to be found in the nucleus as well as the cytoplasm during differentiation, while CUL4A has been reported to be present predominantly in the cytoplasm. CUL4B is suggested to have a more specific role than CUL4A due to the presence of functional nuclear localization signal sequence in the N-terminus. CUL4A is noted as essential for embryonic development (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~10000 FC: ~~ 1:60 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: CUL4A/B Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Neurology, Cell Structure

Restrictions: For Research Use only

Publications

Product: Shiyanov, Nag, Raychaudhuri: "Cullin 4A associates with the UV-damaged DNA-binding protein DDB." in: The Journal of biological chemistry, Vol. 274, Issue 50, pp. 35309-12, 2000 (PubMed).

Liu, Furukawa, Matsumoto et al.: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).