Cyclin B1 (CCNB1) (C-Term) antibody

Details for Product No. ABIN649581
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Antigen
Synonyms ccnb, cycb, cb267, cycb1, wu:fa19g04, wu:fb16d01, wu:fb16e07, wu:fi21c01, ccnb1, MGC53596, CCNB, Ccnb1-rs1, Ccnb1-rs13, CycB1, Cycb-4, Cycb-5, Cycb1-rs1
Epitope
C-Term
(38), (29), (28), (13), (13), (9), (8), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(283), (110), (97), (29), (27), (19), (16), (14), (12), (3), (2), (1), (1), (1)
Host
Rabbit
(237), (65), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(9), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(212), (90), (80), (76), (65), (47), (40), (19), (15), (14), (12), (7), (4), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN649581
450.00 $
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Specificity A synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members.
Alternative Name Cyclin B1
Background Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).
Synonyms: CCNB1, CCNB, G2/mitotic-specific cyclin-B1
Molecular Weight 58 kDA
Gene ID 891
UniProt P14635
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:100
Comment

Background: Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Cyclin B1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Gallant, Nigg: "Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells." in: The Journal of cell biology, Vol. 117, Issue 1, pp. 213-24, 1992 (PubMed).

Morgan: "Cyclin-dependent kinases: engines, clocks, and microprocessors." in: Annual review of cell and developmental biology, Vol. 13, pp. 261-91, 1998 (PubMed).

Dunphy: "The decision to enter mitosis." in: Trends in cell biology, Vol. 4, Issue 6, pp. 202-7, 2004 (PubMed).

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