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Cyclin B1 (CCNB1) (C-Term) antibody

Antigen

Cyclin B1 (CCNB1)
Synonyms CCNB, ccnb, cycb, cb267, cycb1, wu:fa19g04, wu:fb16d01, wu:fb16e07, wu:fi21c01, CycB1, Cycb-4, Cycb-5, MGC18763, MGC90915, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13, CCNB1, ccnb1, MGC53596, MGC76039
Binding Site
Alternatives

C-Term
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649581
Quantity 100ul  (Variants)
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Cyclin B1
Gene ID 891
UniProt P14635
Description Other names: CCNB1, CCNB, G2/mitotic-specific cyclin-B1
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members.
Molecular Weight 58 kDA
Comments

Background: Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000 FC: ~~ 1:100 ICC: ~~ 1:50 IHC: ~~ 1:100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Cyclin B1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure
Restrictions For Research Use only

Publications
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Product Gallant, Nigg: "Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells." in: The Journal of cell biology, Vol. 117, Issue 1, pp. 213-24, 1992 (PubMed).

Morgan: "Cyclin-dependent kinases: engines, clocks, and microprocessors." in: Annual review of cell and developmental biology, Vol. 13, pp. 261-91, 1998 (PubMed).

Dunphy: "The decision to enter mitosis." in: Trends in cell biology, Vol. 4, Issue 6, pp. 202-7, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Cyclin B1 (CCNB1)", type "Antibodies"
Hosts Rabbit (117), Mouse (45)
Reactivities Human (152), Mouse (Murine) (56), Rat (Rattus) (52), Cow (Bovine) (27), Pig (Porcine) (13), Hamster (10), Dog (Canine) (9), Chicken (6), Guinea Pig (6), Horse (Equine) (5), Cat (Feline) (1), Rabbit (1), Sheep (Ovine) (1)
Applications Western Blotting (WB) (89), Immunofluorescence (IF) (70), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (51), ELISA (49), Immunoprecipitation (IP) (42), Immunohistochemistry (IHC) (28), Flow Cytometry (FACS) (16), Immunocytochemistry (ICC) (8), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), PE,Cy5.5 (4), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pSer126 (12), pSer147 (12), C-Term (8), pSer133 (5), pSer128 (3), Internal Region (2), N-Term (2), Ser126 (2), Ser147 (2), AA 1-21 (1), AA 120-131,pSer126 (1), AA 420-433 (1), Center (1), pTyr221 (1), pTyr816,pTyr785 (1)