|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Cyclin B1 (CCNB1) (C-Term) antibody
|Synonyms||CCNB, ccnb, cycb, cb267, cycb1, wu:fa19g04, wu:fb16d01, wu:fb16e07, wu:fi21c01, CycB1, Cycb-4, Cycb-5, MGC18763, MGC90915, Ccnb1-rs1, Cycb1-rs1, Ccnb1-rs13, CCNB1, ccnb1, MGC53596, MGC76039|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||Cyclin B1|
|Description||Other names: CCNB1, CCNB, G2/mitotic-specific cyclin-B1|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in C-terminus of human cyclin B1 was used as immunogen. The antibody does not cross-react with other cyclin family members.|
|Molecular Weight||58 kDA|
Background: Cyclin B1 regulates mitosis in conjunction with Cdc2 kinase to form the Cdc2-cyclin B1 complex also known as maturation promoting factor (MPF) (1-2). The activation of the complex begins when the regulatory subunit, cyclin B1, associates with the catalytic subunit of Cdc2. Cyclin B1 levels rise during S phase and G2, and peak at mitosis. The timing of mitosis can also be influenced by changes in cyclin B1Cdc2 localization. Cyclin B1 is mostly located in the cytoplasm during S and G2 phases and then rapidly accumulates in the nucleus at the onset of prophase (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000 FC: ~~ 1:100 ICC: ~~ 1:50 IHC: ~~ 1:100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Cyclin B1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Gallant, Nigg: "Cyclin B2 undergoes cell cycle-dependent nuclear translocation and, when expressed as a non-destructible mutant, causes mitotic arrest in HeLa cells." in: The Journal of cell biology, Vol. 117, Issue 1, pp. 213-24, 1992 (PubMed).
Morgan: "Cyclin-dependent kinases: engines, clocks, and microprocessors." in: Annual review of cell and developmental biology, Vol. 13, pp. 261-91, 1998 (PubMed).
Dunphy: "The decision to enter mitosis." in: Trends in cell biology, Vol. 4, Issue 6, pp. 202-7, 2004 (PubMed).