|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Cyclin E2 (CCNE2) (C-Term) antibody
|Synonyms||CYCE2, ccne2, MGC53684, MGC132360, cb165, sb:cb165, zgc:86724, CCNE2, cyce2|
Human, Rat (Rattus)
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Alternative name||Cyclin E2|
|Description||Other names: CCNE2, G1/S-specific cyclin-E2|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in C-terminus of human Cyclin E2 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.|
|Molecular Weight||50 kDA|
Background: Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:5000~10000 IP: ~~ 1:150 FC: ~~ 1:40 ICC: ~~ 1:100~1:250 IHC: ~~ 1:50|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Cyclin E2 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure|
|Restrictions||For Research Use only|
Lauper, Beck, Cariou et al.: "Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle." in: Oncogene, Vol. 17, Issue 20, pp. 2637-43, 1998 (PubMed).
Zariwala, Liu, Xiong: "Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins." in: Oncogene, Vol. 17, Issue 21, pp. 2787-98, 1998 (PubMed).
Gudas, Payton, Thukral et al.: "Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 612-22, 1999 (PubMed).
|Hosts||Rabbit (44), Mouse (2)|
|Reactivities||Human (43), Mouse (Murine) (27), Rat (Rattus) (23), Cow (Bovine) (20), Horse (Equine) (18), Pig (Porcine) (18), Cat (Feline) (1), Chicken (1), Dog (Canine) (1), Rabbit (1)|
|Applications||Western Blotting (WB) (30), Immunofluorescence (IF) (21), ELISA (16), Immunohistochemistry (IHC) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (5), Immunoprecipitation (IP) (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (5), N-Term (4), AA 2-15 (3), pSer641 (1), pThr392 (1)|