Cyclin E2 (CCNE2) (C-Term) antibody

Antigen: Cyclin E2 (CCNE2)

Synonyms: CYCE2, ccne2, MGC53684, MGC132360, cb165, sb:cb165, zgc:86724, CCNE2, cyce2

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649588

Additional Information

Alternative name: Cyclin E2

Gene ID: 9134

UniProt: O96020

Description: Other names: CCNE2, G1/S-specific cyclin-E2

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in C-terminus of human Cyclin E2 was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.

Molecular Weight: 50 kDA

Comments:

Background: Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathway(s) during this phase (1). Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both (2). Abnormally high levels of cyclin E expression have frequently been observed in human cancers (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~10000 IP: ~~ 1:150 FC: ~~ 1:40 ICC: ~~ 1:100~1:250 IHC: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Cyclin E2 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Cycle, Cell Structure

Restrictions: For Research Use only

Publications

Product: Lauper, Beck, Cariou et al.: "Cyclin E2: a novel CDK2 partner in the late G1 and S phases of the mammalian cell cycle." in: Oncogene, Vol. 17, Issue 20, pp. 2637-43, 1998 (PubMed).

Zariwala, Liu, Xiong: "Cyclin E2, a novel human G1 cyclin and activating partner of CDK2 and CDK3, is induced by viral oncoproteins." in: Oncogene, Vol. 17, Issue 21, pp. 2787-98, 1998 (PubMed).

Gudas, Payton, Thukral et al.: "Cyclin E2, a novel G1 cyclin that binds Cdk2 and is aberrantly expressed in human cancers." in: Molecular and cellular biology, Vol. 19, Issue 1, pp. 612-22, 1999 (PubMed).