Keratin 7 (KRT7) (C-Term) antibody

Antigen: Keratin 7 (KRT7)

Synonyms: K7, CK7, SCL, K2C7, MGC3625, MGC129731, Krt2-7, MGC11625, D15Wsu77e, KRT7, MGC137771

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649623

Additional Information

Alternative name: Cytokeratin-7

Gene ID: 3855

UniProt: P08729

Description: Other names: KRT7, SCL, Keratin, type II cytoskeletal 7, Sarcolectin

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-terminus of human CK-7 was used as an immunogen.

Molecular Weight: 55 kDA

Comments:

Background: Cytokeratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (CK7) is expressed in a wide range of epithelial structures in humans. The expression pattern in mice is similar to the reported human distribution, with substantial expression observed in lung, bladder, mesothelium, hair follicle, and ductal structures (1). Protein expression levels of CK7 appear significantly higher in chromophobe RCC compared to oncocytoma (p < 0.001). Studies provide further evidence that CK7 and parvalbumin immunostains may be useful in differentiating oncocytoma from chromophobe RCC in problematic cases (2). Also, the pattern of immunohistochemical expression of CK 7 along with CK 20 is commonly used to assess possible primary sites of metastatic carcinomas (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~5000 IP: ~~ 1:50 FC: ~~ 1:20 ICC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Cytokeratin-7 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Smith, Porter, Corden et al.: "Cloning of human, murine, and marsupial keratin 7 and a survey of K7 expression in the mouse." in: Biochemical and biophysical research communications, Vol. 297, Issue 4, pp. 818-27, 2002 (PubMed).

Coons, Estrada, Gamez et al.: "Cytokeratin CK 7 and CK 20 expression in pituitary adenomas." in: Endocrine pathology, Vol. 16, Issue 3, pp. 201-10, 2005 (PubMed).

Adley, Papavero, Sugimura et al.: "Diagnostic value of cytokeratin 7 and parvalbumin in differentiating chromophobe renal cell carcinoma from renal oncocytoma." in: Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology, Vol. 28, Issue 4, pp. 228-36, 2006 (PubMed).