Death-Associated Protein Kinase 2 (DAPK2) (N-Term) antibody

Antigen: Death-Associated Protein Kinase 2 (DAPK2)

Synonyms: DRP1, DRP-1, MGC119312, drp1, drp-1, DAPK2, dapk2

Binding Site: N-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649636

Additional Information

Alternative name: DAPK2

Gene ID: 23604

UniProt: Q9UIK4

Description: Other names: DAPK2, Death-associated protein kinase 2, DAP-kinase-related protein 1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the N-terminus of human DAPK2 was used as an immunogen.

Molecular Weight: 43 kDA

Comments:

Background: Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:15 IHC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: DAPK2 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kawai, Nomura, Hoshino et al.: "Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity." in: Oncogene, Vol. 18, Issue 23, pp. 3471-80, 1999 (PubMed).

Inbal, Shani, Cohen et al.: "Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis." in: Molecular and cellular biology, Vol. 20, Issue 3, pp. 1044-54, 2000 (PubMed).

Shani, Henis-Korenblit, Jona et al.: "Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding." in: The EMBO journal, Vol. 20, Issue 5, pp. 1099-113, 2001 (PubMed).