Death-Associated Protein Kinase 2 (DAPK2) (N-Term) antibody

Details for Product No. ABIN649636
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Antigen
Synonyms drp1, drp-1, DAPK2, dapk2, DRP-1, DRP1
Epitope
N-Term
(25), (17), (17), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(102), (71), (58), (27), (13), (12), (12), (1)
Host
Rabbit
(94), (9), (8)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(68), (47), (37), (32), (20), (9), (3), (1), (1)
Pubmed 3 references available
Quantity 100 µL
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Catalog No. ABIN649636
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Specificity A synthetic peptide corresponding to residues on the N-terminus of human DAPK2 was used as an immunogen.
Alternative Name DAPK2
Background Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).
Synonyms: DAPK2, Death-associated protein kinase 2, DAP-kinase-related protein 1
Molecular Weight 43 kDA
Gene ID 23604
UniProt Q9UIK4
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes IHC: = 1:50-100, WB: = 1:1000-10000
Comment

Background: Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment DAPK2 Antibody (N-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kawai, Nomura, Hoshino et al.: "Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity." in: Oncogene, Vol. 18, Issue 23, pp. 3471-80, 1999 (PubMed).

Inbal, Shani, Cohen et al.: "Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis." in: Molecular and cellular biology, Vol. 20, Issue 3, pp. 1044-54, 2000 (PubMed).

Shani, Henis-Korenblit, Jona et al.: "Autophosphorylation restrains the apoptotic activity of DRP-1 kinase by controlling dimerization and calmodulin binding." in: The EMBO journal, Vol. 20, Issue 5, pp. 1099-113, 2001 (PubMed).

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