Death-Associated Protein Kinase 3 (DAPK3) antibody

Antigen: Death-Associated Protein Kinase 3 (DAPK3)

Synonyms: ZIP, ZIPK, FLJ36473, DAPK3, dapk3, wu:fc25b08, si:dkey-57k2.5, MGC114871, dlk, Dapkl

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649637

Additional Information

Alternative name: DAPK3

Gene ID: 1613

UniProt: O43293

Description: Other names: DAPK3, ZIPK, Death-associated protein kinase 3, ZIP-kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human DAPK3 was used as an immunogen.

Molecular Weight: 52 kDA

Comments:

Background: Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500 FC: ~~ 1:10

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: DAPK3 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kawai, Matsumoto, Takeda et al.: "ZIP kinase, a novel serine/threonine kinase which mediates apoptosis." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1642-51, 1998 (PubMed).

Murata-Hori, Suizu, Iwasaki et al.: "ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells." in: FEBS letters, Vol. 451, Issue 1, pp. 81-4, 1999 (PubMed).