Death-Associated Protein Kinase 3 (DAPK3) antibody

Details for Product No. ABIN649637
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Antigen
Synonyms ZIP, ZIPK, dlk, Dapkl, DAPK3, dapk3, wu:fc25b08, si:dkey-57k2.5, MGC114871
Reactivity
Human
(63), (44), (39), (13), (12), (12)
Host
Rabbit
(66)
Clonality
Monoclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(57), (29), (17), (14), (11), (10), (10), (3), (1), (1)
Pubmed 2 references available
Catalog no. ABIN649637
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Specificity A synthetic peptide corresponding to residues on the C-terminus of human DAPK3 was used as an immunogen.
Alternative Name DAPK3
Background Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).
Synonyms: DAPK3, ZIPK, Death-associated protein kinase 3, ZIP-kinase
Molecular Weight 52 kDA
Gene ID 1613
UniProt O43293
Research Area Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500
Comment

Background: Death-associated protein kinase (DAPK3), which is a mediator of apoptosis, is induced by gamma interferon. DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. HeLa ZIPK phosphorylated the regulatory light chain of myosin II (MRLC) at both serine 19 and threonine 18 in a Ca2+/calmodulin independent manner. It interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment DAPK3 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kawai, Matsumoto, Takeda et al.: "ZIP kinase, a novel serine/threonine kinase which mediates apoptosis." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1642-51, 1998 (PubMed).

Murata-Hori, Suizu, Iwasaki et al.: "ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells." in: FEBS letters, Vol. 451, Issue 1, pp. 81-4, 1999 (PubMed).

Hosts (66)
Reactivities (63), (44), (39), (13), (12), (12)
Applications (57), (29), (17), (14), (11), (10), (10), (3), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1)
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