Death-Associated Protein Kinase 3 (DAPK3) antibody

Antigen: Death-Associated Protein Kinase 3 (DAPK3)

Synonyms: ZIP, ZIPK, FLJ36473, DAPK3, dapk3, wu:fc25b08, si:dkey-57k2.5, MGC114871, dlk, Dapkl

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN649638

Additional Information

Alternative name: DAPK3

Gene ID: 1613

UniProt: O43293

Description: Other names: DAPK3, ZIPK, Death-associated protein kinase 3, ZIP-kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the N-terminus of human DAPK3 was used as an immunogen.

Molecular Weight: 52 kDA

Comments:

Background: DAPK3 (Death-associated protein kinase) is a Ca2+ calmodulin serine/threonine kinase which acts as a positive regulator of apoptosis through its catalytic activities. DAPK3 is a mediator of apoptosis and is induced by gamma interferon (1). Results suggest that DAPK3, in collaboration with Daxx and Par-4, mediates a novel nuclear pathway for apoptosis (2). It has been shown that the nuclear serine/threonine-specific kinase phosphorylates core histones H3 and H4, and myosine light chain in vitro. DAPK3 interacts with transcription and splicing factors as well as with pro-apoptotic protein Par-4 suggesting that it participates in multiple cellular processes (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~10000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: DAPK3 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Signaling, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kawai, Matsumoto, Takeda et al.: "ZIP kinase, a novel serine/threonine kinase which mediates apoptosis." in: Molecular and cellular biology, Vol. 18, Issue 3, pp. 1642-51, 1998 (PubMed).

Murata-Hori, Suizu, Iwasaki et al.: "ZIP kinase identified as a novel myosin regulatory light chain kinase in HeLa cells." in: FEBS letters, Vol. 451, Issue 1, pp. 81-4, 1999 (PubMed).