Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) antibody

Details for Product No. ABIN649639
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Antigen
Synonyms darpp32, darpp-32, Darpp-32, Darpp32, DARPP-32, DARPP32, AU040756
Reactivity
Human, Rat (Rattus)
(143), (121), (109), (51), (49), (36), (36), (12), (4), (4), (4), (3), (1), (1), (1)
Host
Rabbit
(151), (8), (2), (2), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(123), (64), (52), (30), (30), (20), (7), (4), (4), (2), (2), (1), (1)
Pubmed 2 references available
Quantity 100 μL
Options
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Catalog No. ABIN649639
450.00 $
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Specificity A synthetic peptide corresponding to residues near the N-term of human DARPP-32 was used as immunogen.
Alternative Name DARPP-32
Background The 32kDa dopamine and cyclic adenosine 3',,5',-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).
Synonyms: PPP1R1B, DARPP32, Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein,DARPP-32
Molecular Weight 32 kDA
Gene ID 84152
UniProt Q9UD71
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure
Application Notes IHC: = 1:250-500, WB: = 1:1000-10000
Comment

Background: The 32kDa dopamine and cyclic adenosine 3',,5',-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment DARPP-32 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

El-Rifai, Smith, Li et al.: "Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP." in: Cancer research, Vol. 62, Issue 14, pp. 4061-4, 2002 (PubMed).

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