Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (C-Term) antibody

Antigen: Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)

Synonyms: Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649640

Additional Information

Alternative name: DARPP-32

Gene ID: 84152

UniProt: Q9UD71

Description: Other names: PPP1R1B, DARPP32, Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein,DARPP-32

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in the C-term of human DARPP-32 was used as immunogen.

Molecular Weight: 32 kDA

Comments:

Background: The 32kDa dopamine and cyclic adenosine 3',,5',-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:50 ICC: ~~ 1:50~100 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: DARPP-32 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

El-Rifai, Smith, Li et al.: "Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP." in: Cancer research, Vol. 62, Issue 14, pp. 4061-4, 2002 (PubMed).