|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (C-Term) antibody
|Synonyms||Darpp32, AU040756, DARPP-32, DARPP32, FLJ20940, Darpp-32, darpp32, darpp-32, PPP1R1B, MGC139723|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
|2 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: PPP1R1B, DARPP32, Protein phosphatase 1 regulatory subunit 1B, Dopamine- and cAMP-regulated neuronal phosphoprotein,DARPP-32|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues in the C-term of human DARPP-32 was used as immunogen.|
|Molecular Weight||32 kDA|
Background: The 32kDa dopamine and cyclic adenosine 3',,5',-monophosphate-regulated phosphoprotein (DARPP-32) can either function as a serine/threonine kinase or as a serine/threonine phosphatase depending upon which particular amino acid residue is phosphorylated (1). When phosphorylated by protein kinase A (PKA) at threonine 34, DARPP-32 becomes a potent inhibitor of protein phosphatase-1 (2-3). Alternatively, when phosphorylated at threonine 75 by cyclin-dependent kinase 5(Cdk5), DARPP-32 is converted into an inhibitor of PKA (1). DARPP-32 and truncated DARPP (t-DARPP) are overexpressed in gastric cancers (4).
|Application Notes||The suggested dilution is: WB: ~~ 1:500~1000 IP: ~~ 1:50 ICC: ~~ 1:50~100 IHC: ~~ 1:250~500|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||DARPP-32 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Signaling, Phospho-specific antibodies, Protein Modifications, Cell Structure|
|Restrictions||For Research Use only|
Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).
El-Rifai, Smith, Li et al.: "Gastric cancers overexpress DARPP-32 and a novel isoform, t-DARPP." in: Cancer research, Vol. 62, Issue 14, pp. 4061-4, 2002 (PubMed).