DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) antibody

Antigen: DNA (Cytosine-5)-Methyltransferase 1 (DNMT1)

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649647

Additional Information

Alternative name: Dnmt1

Gene ID: 23175

UniProt: P26358

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues at the C-terminus of human Dnmt1 was used as an immunogen.

Molecular Weight: 200 kDA

Comments:

Background: Dnmt1 is one of three families of enzymes (Dnmt1, Dnmt2, Dnmt3 (a and b)) that have a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Abnormal methylation patterns are associated with certain human tumors and developmental abnormalities (1). Dnmt1 is responsible for maintenance of the DNA methylation pattern after DNA replication. Dnmt1 is a large protein and can be subdivided into an N- and a C-terminal part. The C-terminal domain harbours the catalytic center. The N-terminal part of the enzyme comprises several individual domains, and plays a role in intracellular targeting and regulation of the enzymeﾒs activity. Dnmt1 shows a preference for hemimethylated CG sites and the specificity of Dnmt1 for hemimethylated target sites is a key property for that function (2).

Synonyms: AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, Dnmt, Met1, Cxxc9, MTase, Met-1, Dnmt1o, MommeD2, mta, cb983, dmnt1, cb1075, fb05h01, wu:fb05h01, DDM2, DECREASED DNA METHYLATION 2, DMT01, DMT1, DNA METHYLTRANSFERASE, DNA METHYLTRANSFERASE 01, DNA METHYLTRANSFERASE 1, MET1, MET2, methyltransferase 1, METHYLTRANSFERASE 2, METHYLTRANSFERASE I, METI

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:10000~50000 IP: ~~ 1:50 FC: ~~ 1:50 ICC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Dnmt1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Pruitt, Tatusova, Maglott: "NCBI reference sequences (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins." in: Nucleic acids research, Vol. 35, Issue Database issue, pp. D61-5, 2007 (PubMed).

Jeltsch: "On the enzymatic properties of Dnmt1: specificity, processivity, mechanism of linear diffusion and allosteric regulation of the enzyme." in: Epigenetics : official journal of the DNA Methylation Society, Vol. 1, Issue 2, pp. 63-6, 2007 (PubMed).