DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody

Antigen: DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B)

Synonyms: cb633, dnmt3b, MGC152725, DNMT3B, LOC100218113, ICF, M.HsaIIIB

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649648

Additional Information

Alternative name: Dnmt3b

Gene ID: 1789

UniProt: Q9UBC3

Description: Other names: DNMT3B, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Dnmt3b was used as an immunogen.

Molecular Weight: 97 kDA

Comments:

Background: DNA (cytosine-5)-methyltransferase 3B (Dnmt3b) is a DNA methyltransferase that is essential for mammalian development potentially through its transcription and repression activity (1). It is one of three different families of DNA (cytosine-5) methyltransferases, Dnmt1, Dnmt2, Dnmt3 (Dnmt3a and Dnmt3b). Dnmt3a and Dnmt3b form complexes with the major maintenance enzyme Dnmt1 (2). Depletion of Dnmt3b has been shown to induce apoptosis of human cancer cells, and reactivate methylation-silenced gene expression (3). Methylation defects in ICF syndrome may also result from impaired stimulation of Dnmt3b activity (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:50~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Dnmt3b Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Kang, Park, Chung: "Dnmt3b, de novo DNA methyltransferase, interacts with SUMO-1 and Ubc9 through its N-terminal region and is subject to modification by SUMO-1." in: Biochemical and biophysical research communications, Vol. 289, Issue 4, pp. 862-8, 2001 (PubMed).

Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).

Kim, Ni, Kelesoglu et al.: "Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases." in: The EMBO journal, Vol. 21, Issue 15, pp. 4183-95, 2002 (PubMed).