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Details for Product No. ABIN649648

DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B) antibody

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Antigen
Synonyms ICF, ICF1, M.HsaIIIB, cb91, dnmt3bl, dnmt7, sb:cb91, wu:fb16h07, cb633, dnmt3b, DNMT3B, LOC100218113
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Application
»Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 3 references available
Catalog no. ABIN649648
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues in human Dnmt3b was used as an immunogen.
Alternative Name Dnmt3b
Background DNA (cytosine-5)-methyltransferase 3B (Dnmt3b) is a DNA methyltransferase that is essential for mammalian development potentially through its transcription and repression activity (1). It is one of three different families of DNA (cytosine-5) methyltransferases, Dnmt1, Dnmt2, Dnmt3 (Dnmt3a and Dnmt3b). Dnmt3a and Dnmt3b form complexes with the major maintenance enzyme Dnmt1 (2). Depletion of Dnmt3b has been shown to induce apoptosis of human cancer cells, and reactivate methylation-silenced gene expression (3). Methylation defects in ICF syndrome may also result from impaired stimulation of Dnmt3b activity (4).
Synonyms: DNMT3B, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB
Molecular Weight 97 kDA
Gene ID 1789
UniProt Q9UBC3
Research Area Ubiquitin-related antibodies, Translation Factors, Signaling, Protein Modifications, Transcription Factors, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:50~100
Comment

Background: DNA (cytosine-5)-methyltransferase 3B (Dnmt3b) is a DNA methyltransferase that is essential for mammalian development potentially through its transcription and repression activity (1). It is one of three different families of DNA (cytosine-5) methyltransferases, Dnmt1, Dnmt2, Dnmt3 (Dnmt3a and Dnmt3b). Dnmt3a and Dnmt3b form complexes with the major maintenance enzyme Dnmt1 (2). Depletion of Dnmt3b has been shown to induce apoptosis of human cancer cells, and reactivate methylation-silenced gene expression (3). Methylation defects in ICF syndrome may also result from impaired stimulation of Dnmt3b activity (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment Dnmt3b Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Kang, Park, Chung: "Dnmt3b, de novo DNA methyltransferase, interacts with SUMO-1 and Ubc9 through its N-terminal region and is subject to modification by SUMO-1." in: Biochemical and biophysical research communications, Vol. 289, Issue 4, pp. 862-8, 2001 (PubMed).

Beaulieu, Morin, Chute et al.: "An essential role for DNA methyltransferase DNMT3B in cancer cell survival." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28176-81, 2002 (PubMed).

Kim, Ni, Kelesoglu et al.: "Co-operation and communication between the human maintenance and de novo DNA (cytosine-5) methyltransferases." in: The EMBO journal, Vol. 21, Issue 15, pp. 4183-95, 2002 (PubMed).

Alternatives for antigen "DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B)", type "Antibodies"
Hosts (39), (10), (5)
Reactivities (49), (15), (3), (1), (1), (1), (1)
Applications (52), (18), (16), (15), (14), (13), (12), (5), (2), (2), (1)
Epitopes (8), (2), (2), (2), (2), (1), (1), (1), (1)
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