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Eukaryotic Translation Elongation Factor 2 (EEF2) antibody

Antigen

Eukaryotic Translation Elongation Factor 2 (EEF2)
Synonyms eef2, MGC76191, MGC79628, EEF2, DKFZp468K065, DKFZp468M076
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
2 references available
Certificates ISO 9001:2008
Catalog no. ABIN649656
Quantity 100ul
Price 450.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name eEF2
Gene ID 1938
UniProt P13639
Description Other names: EEF2, EF2, Elongation factor 2
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues near the N-terminus of human eEF2 was used as immunogen.
Molecular Weight 95 kDA
Comments

Background: The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:1000~10000 ICC: ~~ 1:50 IHC: ~~ 1:50~100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage eEF2 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Nilsson, Nygård: "Altered sensitivity of eukaryotic elongation factor 2 for trypsin after phosphorylation and ribosomal binding." in: The Journal of biological chemistry, Vol. 266, Issue 16, pp. 10578-82, 1991 (PubMed).

Rapp, Klaudiny, Hagendorff et al.: "Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells." in: Biological chemistry Hoppe-Seyler, Vol. 370, Issue 10, pp. 1071-5, 1990 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Eukaryotic Translation Elongation Factor 2 (EEF2)", type "Antibodies"
Hosts Rabbit (65), Mouse (6)
Reactivities Human (70), Rat (Rattus) (48), Mouse (Murine) (46), Chicken (20), Cow (Bovine) (20), Horse (Equine) (19), Pig (Porcine) (19), Caenorhabditis elegans (C. elegans) (1), Cat (Feline) (1), Dog (Canine) (1), Fruit Fly (Drosophila melanogaster) (1)
Applications Immunofluorescence (IF) (42), Western Blotting (WB) (40), ELISA (28), Immunohistochemistry (IHC) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Flow Cytometry (FACS) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (5), Immunoprecipitation (IP) (5), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), Alexa Flour 488 (1)
Epitopes pThr56 (21), N-Term (5), C-Term (4), C-Term,AA 828-858 (1), N-Term,AA 45-75 (1), pThr56, pThr58 (1)