Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN649658

Eukaryotic Translation Elongation Factor 2 (EEF2) (C-Term) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms Ef-2, EEF-2, EF-2, EF2, eef2, MGC76191, MGC79628, EEF2
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
Pubmed 1 reference available
Catalog no. ABIN649658
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues on the C-terminus of human eEF2 was used as an immunogen.
Alternative Name eEF2
Background The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).
Molecular Weight 95 kDA
Gene ID 1938
UniProt P13639
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:1000 IP: ~~ 1:200 FC: ~~ 1:20 ICC: ~~ 1:100 IHC: ~~ 1:100
Comment

Background: The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment eEF2 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Rapp, Klaudiny, Hagendorff et al.: "Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells." in: Biological chemistry Hoppe-Seyler, Vol. 370, Issue 10, pp. 1071-5, 1990 (PubMed).

Alternatives for antigen "Eukaryotic Translation Elongation Factor 2 (EEF2)", type "Antibodies"
Hosts (72), (11)
Reactivities (82), (54), (44), (12), (12), (12), (1), (1)
Applications (56), (37), (30), (24), (20), (18), (9), (4), (3), (2)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (32), (13), (5), (4), (1)
Beacon