Eukaryotic Translation Elongation Factor 2 (EEF2) (C-Term) antibody

Details for Product No. ABIN649658
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Antigen
Synonyms eef2, MGC76191, MGC79628, EEF2, Dmel\CG4849, EFTUD2, eEF2, EEF-2, EF-2, EF2, Ef-2
Epitope
C-Term
(35), (14), (6), (6), (4), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(99), (56), (46), (12), (12), (12), (2), (2)
Host
Rabbit
(86), (13)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(71), (37), (32), (31), (30), (23), (9), (3), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μL
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Catalog No. ABIN649658
450.00 $
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Specificity A synthetic peptide corresponding to residues on the C-terminus of human eEF2 was used as an immunogen.
Alternative Name eEF2
Background The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).
Molecular Weight 95 kDA
Gene ID 1938
UniProt P13639
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000 , IHC: = 1:100
Comment

Background: The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment eEF2 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Rapp, Klaudiny, Hagendorff et al.: "Complete sequence of the coding region of human elongation factor 2 (EF-2) by enzymatic amplification of cDNA from human ovarian granulosa cells." in: Biological chemistry Hoppe-Seyler, Vol. 370, Issue 10, pp. 1071-5, 1990 (PubMed).

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