Epidermal Growth Factor Receptor (EGFR) antibody

Details for Product No. ABIN649662
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Antigen
Synonyms
C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DER1, DEgfr, Degfr, Der, DmHD-33, Dmel\\CG10079, EFG-R, EGF-R, EGFR, EGFr, EGfr, EK2-6, Egf,  ... show more
C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DER1, DEgfr, Degfr, Der, DmHD-33, Dmel\\CG10079, EFG-R, EGF-R, EGFR, EGFr, EGfr, EK2-6, Egf, Egf-r, EgfR, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, TOP, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR, dEGFR1, dEgfr, der, egfr, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, mor1, top, top/DER, top/flb, torpedo/Egfr, torpedo/egfr, EGFR12, EGFR15, egfr1, Erbb2, ERBB, ERBB1, HER1, PIG61, mENA, ErbB-1, Errp, 9030024J15Rik, AI552599, Erbb, Errb1, Wa5, wa-2, wa2 show less
Reactivity
Human, Rat (Rattus)
(1385), (673), (620), (160), (145), (124), (77), (7), (6), (3), (2), (1)
Host
Rabbit
(1090), (272), (22), (14), (6), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(71), (47), (36), (26), (25), (25), (25), (25), (25), (25), (25), (16), (10), (10), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(916), (424), (290), (249), (232), (158), (138), (120), (75), (67), (53), (18), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 100 µL
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Catalog No. ABIN649662
450.00 $
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Specificity A synthetic peptide corresponding to residues near C-terminus of human EGF receptor was used as immunogen.
Alternative Name EGFR
Background Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal of EGFR (3). Autophosphorylation of Tyr 992 creates a binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain, inducing downstream signaling (4).
Synonyms: EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1
Molecular Weight 170-180 kDA
Gene ID 1956
UniProt P00533
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes The suggested dilution is: WB: = 1:1000-10000 , IHC: = 1:10-25
Comment

Background: Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal of EGFR (3). Autophosphorylation of Tyr 992 creates a binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain, inducing downstream signaling (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment EGFR Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hunter: "The epidermal growth factor receptor gene and its product." in: Nature, Vol. 311, Issue 5985, pp. 414-6, 1984 (PubMed).

Reynolds, Todaro, Fryling et al.: "Human transforming growth factors induce tyrosine phosphorylation of EGF receptors." in: Nature, Vol. 292, Issue 5820, pp. 259-62, 1981 (PubMed).

Emlet, Moscatello, Ludlow et al.: "Subsets of epidermal growth factor receptors during activation and endocytosis." in: The Journal of biological chemistry, Vol. 272, Issue 7, pp. 4079-86, 1997 (PubMed).

Bishayee, Beguinot, Bishayee: "Phosphorylation of tyrosine 992, 1068, and 1086 is required for conformational change of the human epidermal growth factor receptor c-terminal tail." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 525-36, 1999 (PubMed).

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