anti-EGFR antibody (Epidermal Growth Factor Receptor)

Details for Product anti-EGFR Antibody No. ABIN649662
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Antigen
Reactivity
Human, Rat (Rattus)
(1808), (698), (635), (13), (7), (6), (5), (3), (2), (2), (2), (1), (1)
Host
Rabbit
(1323), (426), (41), (25), (8), (3), (1)
Clonality
Monoclonal
Conjugate
This EGFR antibody is un-conjugated
(85), (61), (47), (34), (27), (26), (24), (22), (22), (22), (22), (22), (22), (22), (2), (2), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(1254), (551), (386), (293), (241), (220), (182), (151), (77), (72), (70), (52), (30), (19), (18), (11), (6), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
Options
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Specificity A synthetic peptide corresponding to residues near C-terminus of human EGF receptor was used as immunogen.
Alternative Name EGFR (EGFR Antibody Abstract)
Background Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal of EGFR (3). Autophosphorylation of Tyr 992 creates a binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain, inducing downstream signaling (4).
Synonyms: EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1
Molecular Weight 170-180 kDA
Gene ID 1956
UniProt P00533
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Pathways NF-kappaB Signaling
Application Notes The suggested dilution is: WB: = 1:1000-10000 , IHC: = 1:10-25
Comment

Background: Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal of EGFR (3). Autophosphorylation of Tyr 992 creates a binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain, inducing downstream signaling (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment EGFR Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Bishayee, Beguinot, Bishayee: "Phosphorylation of tyrosine 992, 1068, and 1086 is required for conformational change of the human epidermal growth factor receptor c-terminal tail." in: Molecular biology of the cell, Vol. 10, Issue 3, pp. 525-36, 1999 (PubMed).

Emlet, Moscatello, Ludlow et al.: "Subsets of epidermal growth factor receptors during activation and endocytosis." in: The Journal of biological chemistry, Vol. 272, Issue 7, pp. 4079-86, 1997 (PubMed).

Reynolds, Todaro, Fryling et al.: "Human transforming growth factors induce tyrosine phosphorylation of EGF receptors." in: Nature, Vol. 292, Issue 5820, pp. 259-62, 1981 (PubMed).

Hunter: "The epidermal growth factor receptor gene and its product." in: Nature, Vol. 311, Issue 5985, pp. 414-6, 1984 (PubMed).

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Catalog No. ABIN649662
481.43 $
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