Did you know that you can buy products from over 140 different suppliers from us?

Epidermal Growth Factor Receptor (EGFR) (pTyr845) antibody

Details for Product No. ABIN649676
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, 9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2, ErbB-1, c-erbB, C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top ... show more
ERBB, HER1, mENA, ERBB1, PIG61, 9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2, ErbB-1, c-erbB, C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DER1, DEgfr, Degfr, Der, DmHD-33, Dmel\\CG10079, EFG-R, EGF-R, EGFR, EGFr, EGfr, EK2-6, Egf, Egf-r, EgfR, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, TOP, Torpedo/DER, Torpedo/Egfr, d-egf-r, dEGFR, dEGFR1, dEgfr, der, egfr, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, mor1, top, top/DER, top/flb, torpedo/Egfr, torpedo/egfr, EGFR12, EGFR15, egfr1, Erbb2 show less
Epitope
pTyr845
(88), (55), (51), (45), (44), (41), (40), (39), (39), (39), (32), (26), (24), (23), (20), (17), (17), (17), (15), (15), (13), (13), (13), (13), (13), (13), (12), (12), (12), (9), (9), (9), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(1338), (666), (615), (162), (147), (124), (79), (7), (6), (4), (1), (1)
Host
Rabbit
(1068), (251), (22), (13), (6), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(70), (42), (35), (26), (25), (25), (25), (25), (25), (25), (25), (13), (10), (10), (2), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(902), (456), (294), (249), (223), (154), (116), (113), (71), (59), (54), (18), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Catalog No. ABIN649676
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 845 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on tyrosine 845
Alternative Name EGFR
Background Epidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. Analysis of predicted secondary structure of the EGF receptor reveals that all of the phosphorylation sites are located near beta turns (3). I t has been reported that stimulating human glioblastoma cells with recombinant HGF induces biologically relevant EGFR activation. EGFR phosphorylation at Tyr(845) and Tyr(1068) increased 6 to 24 h after cell stimulation with HGF and temporally coincided with the induction of transforming growth factor-alpha (~5-fold) and HB-EGF (~23-fold) expression. Tyr(845) and Tyr(1068) phosphorylation, in response to HGF, was inhibited by cycloheximide and actinomycin D, consistent with a requirement for DNA transcription and RNA translation (4).
Synonyms: EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1
Molecular Weight 175 kDA
Gene ID 1956
UniProt P00533
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes The suggested dilution is: WB: = 1:500-1000
Comment

Background: Epidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. Analysis of predicted secondary structure of the EGF receptor reveals that all of the phosphorylation sites are located near beta turns (3). I t has been reported that stimulating human glioblastoma cells with recombinant HGF induces biologically relevant EGFR activation. EGFR phosphorylation at Tyr(845) and Tyr(1068) increased 6 to 24 h after cell stimulation with HGF and temporally coincided with the induction of transforming growth factor-alpha (~5-fold) and HB-EGF (~23-fold) expression. Tyr(845) and Tyr(1068) phosphorylation, in response to HGF, was inhibited by cycloheximide and actinomycin D, consistent with a requirement for DNA transcription and RNA translation (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment EGFR Antibody Phospho (pY845) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hunter: "The epidermal growth factor receptor gene and its product." in: Nature, Vol. 311, Issue 5985, pp. 414-6, 1984 (PubMed).

Reznik, Sang, Ma et al.: "Transcription-dependent epidermal growth factor receptor activation by hepatocyte growth factor." in: Molecular cancer research : MCR, Vol. 6, Issue 1, pp. 139-50, 2008 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN649676
507.14 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
Did you look for something else?
back to top