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Details for Product No. ABIN649676

Epidermal Growth Factor Receptor (EGFR) (pTyr845) antibody

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Antigen
Synonyms
ERBB, HER1, mENA, ERBB1, PIG61, 9030024J15Rik, AI552599, Erbb, Errb1, Errp, Wa5, wa-2, wa2, ErbB-1, c-erbB, C-erb, CG10079, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top ... show more
Epitope
»Alternatives pTyr845
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
Pubmed 2 references available
Catalog no. ABIN649676
Quantity 100 µL
Price
507.14 $   Plus shipping costs $45.00
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Specificity A phospho specific peptide corresponding to residues surrounding tyrosine 845 of human EGFR was used as an immunogen. This antibody detects EGFR phosphorylated on tyrosine 845
Alternative Name EGFR
Background Epidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. Analysis of predicted secondary structure of the EGF receptor reveals that all of the phosphorylation sites are located near beta turns (3). I t has been reported that stimulating human glioblastoma cells with recombinant HGF induces biologically relevant EGFR activation. EGFR phosphorylation at Tyr(845) and Tyr(1068) increased 6 to 24 h after cell stimulation with HGF and temporally coincided with the induction of transforming growth factor-alpha (~5-fold) and HB-EGF (~23-fold) expression. Tyr(845) and Tyr(1068) phosphorylation, in response to HGF, was inhibited by cycloheximide and actinomycin D, consistent with a requirement for DNA transcription and RNA translation (4).
Synonyms: EGFR, ERBB1, Epidermal growth factor receptor, Receptor tyrosine-protein kinase ErbB-1
Molecular Weight 175 kDA
Gene ID 1956
UniProt P00533
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Cancer, Signaling, Chromatin
Application Notes The suggested dilution is: WB: ~~ 1:500~1000 ICC: ~~ 1:100
Comment

Background: Epidermal growth factor receptor (EGFR) is a 175 kDa transmembrane glycoprotein receptor tyrosine kinase that, activated by epidermal growth factor (EGF), affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The epidermal growth factor (EGF) receptor is regulated by EGF-stimulated autophosphorylation and by phorbol ester-stimulated, protein kinase C (Ca2+/phospholipid-dependent enzyme) mediated phosphorylation at identified sites. Analysis of predicted secondary structure of the EGF receptor reveals that all of the phosphorylation sites are located near beta turns (3). I t has been reported that stimulating human glioblastoma cells with recombinant HGF induces biologically relevant EGFR activation. EGFR phosphorylation at Tyr(845) and Tyr(1068) increased 6 to 24 h after cell stimulation with HGF and temporally coincided with the induction of transforming growth factor-alpha (~5-fold) and HB-EGF (~23-fold) expression. Tyr(845) and Tyr(1068) phosphorylation, in response to HGF, was inhibited by cycloheximide and actinomycin D, consistent with a requirement for DNA transcription and RNA translation (4).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment EGFR Antibody Phospho (pY845) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hunter: "The epidermal growth factor receptor gene and its product." in: Nature, Vol. 311, Issue 5985, pp. 414-6, 1984 (PubMed).

Reznik, Sang, Ma et al.: "Transcription-dependent epidermal growth factor receptor activation by hepatocyte growth factor." in: Molecular cancer research : MCR, Vol. 6, Issue 1, pp. 139-50, 2008 (PubMed).

Alternatives for antigen "Epidermal Growth Factor Receptor (EGFR)", type "Antibodies"
Hosts (962), (246), (26), (15), (4), (1), (1)
Reactivities (1234), (646), (600), (164), (152), (125), (79), (7), (3), (2), (1)
Applications (795), (393), (303), (250), (189), (181), (121), (106), (75), (55), (25), (19), (15), (4), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Conjugates (62), (33), (27), (25), (25), (25), (25), (25), (25), (25), (25), (6), (2), (1), (1), (1), (1)
Epitopes (69), (47), (43), (42), (39), (38), (37), (37), (36), (31), (28), (22), (20), (19), (17), (17), (15), (15), (15), (13), (13), (13), (13), (13), (12), (12), (10), (10), (10), (9), (7), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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