Eukaryotic Translation Initiation Factor 2B, Subunit 1 alpha (EIF2B1) (pSer51) antibody

Antigen: Eukaryotic Translation Initiation Factor 2B, Subunit 1 alpha (EIF2B1)

Binding Site: pSer51

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649677

Additional Information

Alternative name: eIF-2a

Gene ID: 1965

UniProt: P05198

Description: Other names: EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a was used as immunogen. The antibody only detects eIF-2a phosphorylated on Serine 51. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology.

Molecular Weight: 36 kDA

Comments:

Background: Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis (1-3). Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51 (3). Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection (3).

Application Details

Application Notes: The suggested dilution is: ICC: ~~ 1:100~1:250 IHC: ~~ 1:50~1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: eIF-2a Antibody Phospho (pS51) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: de Haro, Méndez, Santoyo: "The eIF-2alpha kinases and the control of protein synthesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 12, pp. 1378-87, 1996 (PubMed).

Kimball: "Eukaryotic initiation factor eIF2." in: The international journal of biochemistry & cell biology, Vol. 31, Issue 1, pp. 25-9, 1999 (PubMed).