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Eukaryotic Translation Initiation Factor 2B, Subunit 1 alpha (EIF2B1) (pSer51) antibody
Human, Rat (Rattus)
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
|2 references available|
|Price||507.14 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: EIF2S1, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic phospho-peptide corresponding to residues surrounding Ser51 of human eIF-2a was used as immunogen. The antibody only detects eIF-2a phosphorylated on Serine 51. Predicted to cross-react with mouse, rat, bovine and pig, based on sequence homology.|
|Molecular Weight||36 kDA|
Background: Eukaryotic initiation factor 2 (eIF2) plays a central role in initiating translation, and provides for a rate-limiting step in protein synthesis. Phosphorylation of a-subunit of eIF2 effectively prevents formation of the eIF2/GTP/Met-tRNAi complex and inhibits global protein synthesis (1-3). Three distinct protein kinases regulate protein synthesis in eukaryotic cells by phosphorylating the a-subunit of eIF2 at Serine51 (3). Phosphorylation occurs under a wide variety of different stimuli, including heat shock, serum deprivation, glucose starvation, amino acid starvation, exposure to heavy metal ions, and viral infection (3).
|Application Notes||The suggested dilution is: ICC: ~~ 1:100~1:250 IHC: ~~ 1:50~1:100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||eIF-2a Antibody Phospho (pS51) can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
de Haro, Méndez, Santoyo: "The eIF-2alpha kinases and the control of protein synthesis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 10, Issue 12, pp. 1378-87, 1996 (PubMed).
Kimball: "Eukaryotic initiation factor eIF2." in: The international journal of biochemistry & cell biology, Vol. 31, Issue 1, pp. 25-9, 1999 (PubMed).