ELK1, Member of ETS Oncogene Family (ELK1) (C-Term) antibody

Details for Product No. ABIN649686
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Antigen
Epitope
C-Term
(67), (44), (32), (26), (8), (7), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(325), (171), (166), (8), (3), (2), (2), (2), (1)
Host
Rabbit
(294), (35)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2)
Application
Western Blotting (WB)
(212), (144), (101), (43), (42), (40), (37), (6), (4), (4), (1), (1), (1)
Pubmed 4 references available
Quantity 100 μL
Options
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Specificity A synthetic peptide corresponding to residues in C-terminus of human ELK-1 was used as immunogen. The antibody does not cross-react with other ETS family members.
Alternative Name Elk-1 (ELK1 Antibody Abstract)
Background Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors (2). Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation.
Synonyms: ELK1, ETS domain-containing protein Elk-1
Molecular Weight 62 kDA
Gene ID 2002
UniProt P19419
Research Area Ubiquitin-related antibodies, Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000
Comment

Background: Elk-1 is a transcription factor that is part of the ets oncogene superfamily (1). Elk-1 forms a ternary complex with the serum response factors, SRE and SRF, mediating gene activity in response to serum and growth factors (2). Activation by several mitogen-activated protein kinases (Erk1 and Erk2) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli (3,4). Serines 383 and 389 in the C-terminal region are the preferred sites for phosphorylation-induced activation.

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Elk-1 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Harindranath, Mills, Mitchell et al.: "The human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the IgH locus." in: Gene, Vol. 221, Issue 2, pp. 215-24, 1998 (PubMed).

Marais, Wynne, Treisman: "The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domain." in: Cell, Vol. 73, Issue 2, pp. 381-93, 1993 (PubMed).

Gille, Kortenjann, Thomae et al.: "ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation." in: The EMBO journal, Vol. 14, Issue 5, pp. 951-62, 1995 (PubMed).

Rao, Huebner, Isobe et al.: "elk, tissue-specific ets-related genes on chromosomes X and 14 near translocation breakpoints." in: Science (New York, N.Y.), Vol. 244, Issue 4900, pp. 66-70, 1989 (PubMed).

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Catalog No. ABIN649686
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