Endonuclease G (ENDOG) (C-Term) antibody

Antigen: Endonuclease G (ENDOG)

Synonyms: FLJ27463, CG8862, DmelCG8862, ENDOG, Tb08.10J17.140, Tb08.10J17.90, TBC1D13, endog

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN649691

Additional Information

Alternative name: Endo G

Gene ID: 2021

UniProt: Q14249

Description: Other names: ENDOG, Endonuclease G, mitochondrial

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human Endo G was used as an immunogen.

Molecular Weight: 35 kDA

Comments:

Background: Endonuclease G (endoG) is a mitochondrion-specific nuclease that translocates to the nucleus during apoptosis. Once released from mitochondria, endoG cleaves chromatin DNA into nucleosomal fragments independently of caspases. Therefore, endoG represents a caspase-independent apoptotic pathway initiated from the mitochondria (1). Endonuclease G (EndoG) is a nuclear-encoded mitochondrial protein reported to be important for both nuclear DNA fragmentation during apoptosis and mitochondrial DNA replication. Findings indicate that EndoG is essential during early embryogenesis and plays a critical role in normal apoptosis and nuclear DNA fragmentation (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:2000~5000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Endo G Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Transcription Factors, Signaling, Metabolism, Cell Structure

Restrictions: For Research Use only

Publications

Product: Li, Luo, Wang: "Endonuclease G is an apoptotic DNase when released from mitochondria." in: Nature, Vol. 412, Issue 6842, pp. 95-9, 2001 (PubMed).

Zhang, Dong, Li et al.: "Endonuclease G is required for early embryogenesis and normal apoptosis in mice." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 26, pp. 15782-7, 2003 (PubMed).