Erythropoietin Receptor (EPOR) (pTyr485) antibody

Details for Product No. ABIN649696
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Antigen
Synonyms EPOR, epor, EPO-R
Epitope
pTyr485
(12), (10), (7), (7), (6), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(116), (46), (41), (14), (12), (1), (1)
Host
Rabbit
(93), (21), (8), (1), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(92), (67), (25), (19), (16), (10), (9), (8), (7), (6), (2), (1), (1), (1)
Pubmed 2 references available
Quantity 100 μL
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Catalog No. ABIN649696
507.14 $
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Specificity A phospho specific peptide corresponding to residues surrounding Tyrosine 485 of human EpoR was used as an immunogen. The antibody only detects EpoR phosphorylated at Tyrosine 485.
Alternative Name EpoR
Background Erythropoietin (Epo) is a hematopoietic cytokine that is a potent regulator of the viability, differentiation and proliferation of erythroid progenitor cells (1, 3). Epo acts on its target cells by inducing homodimerization of the erythropoietin receptor (EpoR), thereby triggering intracellular signaling cascades. The EpoR encompasses eight tyrosine motifs on its cytoplasmic tail that have been shown to recruit a number of regulatory proteins (1). EpoR shares conserved features in its extracellular and cytoplasmic domains with other family members of cytokine and growth factor receptors (2). Alterations of the Epo/EpoR system have recently been shown to be involved in the pathogenesis of familial erythrocytosis and polycythaemia vera (PV) (3).
Synonyms: EPOR, Erythropoietin receptor
Molecular Weight 59 kDA
Gene ID 2057
UniProt P19235
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Application Notes The suggested dilution is: WB: = 1:10000-50000
Comment

Background: Erythropoietin (Epo) is a hematopoietic cytokine that is a potent regulator of the viability, differentiation and proliferation of erythroid progenitor cells (1, 3). Epo acts on its target cells by inducing homodimerization of the erythropoietin receptor (EpoR), thereby triggering intracellular signaling cascades. The EpoR encompasses eight tyrosine motifs on its cytoplasmic tail that have been shown to recruit a number of regulatory proteins (1). EpoR shares conserved features in its extracellular and cytoplasmic domains with other family members of cytokine and growth factor receptors (2). Alterations of the Epo/EpoR system have recently been shown to be involved in the pathogenesis of familial erythrocytosis and polycythaemia vera (PV) (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment EpoR Antibody Phospho (pY485) can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Middleton, Johnson, Jin et al.: "Identification of a critical ligand binding determinant of the human erythropoietin receptor. Evidence for common ligand binding motifs in the cytokine receptor family." in: The Journal of biological chemistry, Vol. 271, Issue 24, pp. 14045-54, 1996 (PubMed).

Hörtner, Nielsch, Mayr et al.: "A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor." in: European journal of biochemistry / FEBS, Vol. 269, Issue 10, pp. 2516-26, 2002 (PubMed).

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