Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Mitogen-Activated Protein Kinase 3 (MAPK3) (C-Term) antibody

Antigen

Mitogen-Activated Protein Kinase 3 (MAPK3)
Synonyms ERK1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, MGC20180, zERK1, fi06b09, wu:fi06b09, Tb08.10J17.940
Binding Site
Alternatives

C-Term
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB)
3 references available
Certificates ISO 9001:2008
Catalog no. ABIN649708
Quantity 100ul  (Variants)
Price 392.86 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 2 to 3 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name Erk1/p44 MAPK
Gene ID 5595
UniProt P27361
Description Other names: MAPK3, ERK1, PRKM3, Mitogen-activated protein kinase 3, p44-ERK1,ERT2,p44-MAPK,Microtubule-associated protein 2 kinase
Characteristics Rabbit Monoclonal Antibody
Specificity A synthetic peptide corresponding to residues in C-terminus of human Erk1 was used as immunogen. The antibody can detect Erk1 and is predicted to react with Erk1c based on sequence homology.
Molecular Weight 44 kDA
Comments

Background: p44 MAP Kinase (Erk1) is a member of a family of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth and differentiation (1,2). Erk1 regulates meiosis and mitosis by phosphorylating a number of transcription factors such as ELK-1. Activation of Erk1 occurs through phosphorylation of threonine 202 and tyrosine 204 (of human MAP kinase) (3). Erk1 is also known to autophosphorylate on tyrosine (3).

Application Details
Publications Alternatives Variants back to top

Application Notes The suggested dilution is: WB: ~~ 1:1000
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Erk1/p44 MAPK Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Chromatin, Cell Structure
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Payne, Rossomando, Martino et al.: "Identification of the regulatory phosphorylation sites in pp42/mitogen-activated protein kinase (MAP kinase)." in: The EMBO journal, Vol. 10, Issue 4, pp. 885-92, 1991 (PubMed).

Charest, Mordret, Harder et al.: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

Marshall: "Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation." in: Cell, Vol. 80, Issue 2, pp. 179-85, 1995 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Mitogen-Activated Protein Kinase 3 (MAPK3)", type "Antibodies"
Hosts Rabbit (169), Mouse (27), Goat (7), Chicken (1), Sheep (1)
Reactivities Human (191), Rat (Rattus) (118), Mouse (Murine) (100), Cow (Bovine) (22), Dog (Canine) (20), Chicken (19), Pig (Porcine) (16), Guinea Pig (15), Horse (Equine) (15), Xenopus laevis (5), Fruit Fly (Drosophila melanogaster) (2), Monkey (2), Rabbit (2), Sheep (Ovine) (2), Cat (Feline) (1), Primate (1), Zebrafish (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (143), ELISA (82), Immunofluorescence (IF) (62), Immunohistochemistry (IHC) (40), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35), Immunoprecipitation (IP) (29), Immunocytochemistry (ICC) (13), Flow Cytometry (FACS) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (DB) (3), Dot Blot (Dot) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Functional Studies (Func) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), FITC (4), PE,Cy5.5 (4), HRP (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr204 (15), C-Term (12), pThr202 (9), Internal Region (8), pThr202, pTyr204 (8), N-Term (6), Tyr204 (5), Thr202 (4), pThr202, pThr204 (4), pThr202,pThr197 (4), pTyr204,pThr202 (4), Asp192 (2), pThr185, pTyr187 (2), AA 174-183 (1), AA 324-345 (1), AA 34-0 (1), AA 350-400 (1), C-Term,AA 365-379 (1), Tyr202 (1), pSer518 (1), pT-E-pY Motif (1), pThr431 (1), pTyr1068 (1)