Mitogen-Activated Protein Kinase 1 (MAPK1) (C-Term) antibody

Antigen: Mitogen-Activated Protein Kinase 1 (MAPK1)

Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, Erk2, Prkm1, C78273, p42mapk, AA407128, AU018647, 9030612K14Rik, zERK2, MAPK1, Xp42, erk, erk2, mapk, mpk1, xp42, mapk1a, MGC68934, ert1, mapk2, prkm1, prkm2, F14N23.9, F14N23_9, MITOGEN-ACTIVATED PROTEIN KINASE, MITOGEN-ACTIVATED PROTEIN KINASE 1, MPK1

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649710

Additional Information

Alternative name: Erk2/p42 MAPK

Gene ID: 5594

UniProt: P28482

Description: Other names: MAPK1, ERK2, PRKM1, PRKM2, Mitogen-activated protein kinase 1, p42-MAPK,ERT1

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues near the C-term of human ERK2 p42 MAPK was used as immunogen.

Molecular Weight: 42 kDA

Comments:

Background: Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen activated protein kinase (MAPK) is involved in eukaryotic signal transduction (1) . Activated by a dual phosphorylation on threonine and tyrosine residue by the dual-specificity kinase MKK1/2, ERK2 p42 translocate to the nucleus after stimulation (2). Once phosphorylated, ERK2 can form dimers with phosphorylated and unphosphorylated ERK2 partners. It is also able to phosphorylate multiple substrates such as EGFR, c-PLA2, Elk1/TCF and c-Myc (3-5).

Synonyms: ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, Erk2, Prkm1, C78273, p42mapk, AA407128, AU018647, 9030612K14Rik, zERK2, MAPK1, Xp42, erk, erk2, mapk, mpk1, xp42, mapk1a, MGC68934, ert1, mapk2, prkm1, prkm2, F14N23.9, F14N23_9, MITOGEN-ACTIVATED PROTEIN KINASE, MITOGEN-ACTIVATED PROTEIN KINASE 1, MPK1

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~5000 IP: ~~ 1:20 FC: ~~ 1:60 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Erk2/p42 MAPK Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Seth, Gonzalez, Gupta et al.: "Signal transduction within the nucleus by mitogen-activated protein kinase." in: The Journal of biological chemistry, Vol. 267, Issue 34, pp. 24796-804, 1992 (PubMed).

Lin, Wartmann, Lin et al.: "cPLA2 is phosphorylated and activated by MAP kinase." in: Cell, Vol. 72, Issue 2, pp. 269-78, 1993 (PubMed).