Ezh antibody

Details for Product No. ABIN649716
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Antigen
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Application
Western Blotting (WB), Immunofluorescence (IF)
Pubmed 3 references available
Quantity 100 μL
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Catalog No. ABIN649716
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Specificity A synthetic peptide corresponding to residues near the C-terminus of human Ezh 1/2 was used as an immunogen.
Background Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression.(2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3).
Synonyms: EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, Enhancer of zeste homolog 2,ENX-1,Lysine N-methyltransferase 6
Molecular Weight 91 kDA
UniProt Q15910
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure
Application Notes WB: = 1:1000-2000
Comment

Background: Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression.(2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Ezh Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Hobert, Jallal, Ullrich: "Interaction of Vav with ENX-1, a putative transcriptional regulator of homeobox gene expression." in: Molecular and cellular biology, Vol. 16, Issue 6, pp. 3066-73, 1996 (PubMed).

Su, Basavaraj, Krutchinsky et al.: "Ezh2 controls B cell development through histone H3 methylation and Igh rearrangement." in: Nature immunology, Vol. 4, Issue 2, pp. 124-31, 2003 (PubMed).

Cha, Zhou, Xia et al.: "Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3." in: Science (New York, N.Y.), Vol. 310, Issue 5746, pp. 306-10, 2005 (PubMed).

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