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Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)
|3 references available|
|Price||450.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Description||Other names: EZH2, KMT6, Histone-lysine N-methyltransferase EZH2, Enhancer of zeste homolog 2,ENX-1,Lysine N-methyltransferase 6|
|Characteristics||Rabbit Monoclonal Antibody|
|Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Ezh 1/2 was used as an immunogen.|
|Molecular Weight||91 kDA|
Background: Enhancer of Zeste homolog 2 (Ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone H3. It has been shown that Akt phosphorylates Ezh2 at serine 21 and suppresses its methyltransferase activity by impeding Ezh2 binding to histone H3, which results in a decrease of lysine 27 trimethylation and derepression of silenced genes (1). Ezh2 interacts specifically with Vav both in vitro and in vivo. ENX-1 represents the human homolog of the Drosophila Enhancer of zeste gene, a member of the Polycomb group of genes, which are transcriptional regulators of homeobox gene expression.(2). Ezh2 is an essential epigenetic regulator of embryonic development in mice, but its role in the adult organism is unknown. High expression of Ezh2 in developing murine lymphocytes suggests Ezh2 involvement in lymphopoiesis. Data suggest Ezh2-dependent histone H3 methylation as a novel regulatory mechanism controlling Igh rearrangement during early murine B cell development (3).
|Application Notes||The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:30 FC: ~~ 1:40 ICC: ~~ 1:50~100|
|Buffer||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Ezh Antibody can be stored at -20°C for up to 12 months from time of receipt.|
|Research Area||Translation Factors, Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Chromatin, Cell Structure|
|Restrictions||For Research Use only|
Hobert, Jallal, Ullrich: "Interaction of Vav with ENX-1, a putative transcriptional regulator of homeobox gene expression." in: Molecular and cellular biology, Vol. 16, Issue 6, pp. 3066-73, 1996 (PubMed).
Su, Basavaraj, Krutchinsky et al.: "Ezh2 controls B cell development through histone H3 methylation and Igh rearrangement." in: Nature immunology, Vol. 4, Issue 2, pp. 124-31, 2003 (PubMed).
Cha, Zhou, Xia et al.: "Akt-mediated phosphorylation of EZH2 suppresses methylation of lysine 27 in histone H3." in: Science (New York, N.Y.), Vol. 310, Issue 5746, pp. 306-10, 2005 (PubMed).