F1H1 antibody

Antigen: F1H1

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN649721

Additional Information

Gene ID: 55662

UniProt: Q9NWT6

Description: Other names: HIF1AN, FIH1, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase,Factor inhibiting HIF-1

Characteristics: Rabbit Monoclonal

Specificity: A synthetic peptide corresponding to residues in human FIH1 was used as an immunogen.

Molecular Weight: 40 kDA

Comments:

Background: Factor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000~20000 FC: ~~ 1:100 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: F1H1 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Signaling, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Publications

Product: Mahon, Hirota, Semenza: "FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity." in: Genes & development, Vol. 15, Issue 20, pp. 2675-86, 2001 (PubMed).

Lando, Peet, Gorman et al.: "FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor." in: Genes & development, Vol. 16, Issue 12, pp. 1466-71, 2002 (PubMed).

Zheng, Linke, Dias et al.: "Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3368-73, 2008 (PubMed).