PTK2B Protein tyrosine Kinase 2 beta (PTK2B) (C-Term) antibody

Antigen: PTK2B Protein tyrosine Kinase 2 beta (PTK2B)

Binding Site: C-Term

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649734

Additional Information

Alternative name: FAK2/Pyk2

Gene ID: 2185

UniProt: Q14289

Description: Other names: PTK2B, FAK2, PYK2, RAFTK, Protein tyrosine kinase 2 beta, Related adhesion focal tyrosine kinase

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues on the C-terminus of human FAK2 was used as an immunogen.

Molecular Weight: 116 kDA

Comments:

Background: Focal adhesion kinase 2 (FAK2), which is highly expressed in the central nervous system, is rapidly phosphorylated on tyrosine residues in response to various stimuli that elevate the intracellular calcium concentration, as well as by protein kinase C activation. Activation of FAK2 leads to modulation of ion channel function and activation of the MAP kinase signalling pathway. FAK2 activation may provide a mechanism for a variety of short- and long-term calcium-dependent signalling events in the nervous system (1). FAK2 is activated by a variety of G-protein-coupled receptors and by extracellular signals that elevate intracellular Ca2+ concentration. Pap has been identified as a new Pyk2 binding protein. It has been demonstrated that Pap forms a stable complex with Pyk2 and that activation of Pyk2 leads to tyrosine phosphorylation of Pap in living cells. Immunofluorescence experiments demonstrate that Pap is localized in the Golgi apparatus and at the plasma membrane, where it is colocalized with Pyk2. Pap functions as a GAP for Arf and that Pyk2 may be involved in regulation of vesicular transport through its interaction with Pap (2).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~2000 IP: ~~ 1:50 IHC: ~~ 1:100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: FAK2/Pyk2 Antibody (C-term) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Ubiquitin-related antibodies, Phospho-specific antibodies, Protein Modifications, Cell Structure, Signaling, Chromatin

Restrictions: For Research Use only

Publications

Product: Lev, Moreno, Martinez et al.: "Protein tyrosine kinase PYK2 involved in Ca(2+)-induced regulation of ion channel and MAP kinase functions." in: Nature, Vol. 376, Issue 6543, pp. 737-45, 1995 (PubMed).

Andreev, Simon, Sabatini et al.: "Identification of a new Pyk2 target protein with Arf-GAP activity." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2338-50, 1999 (PubMed).