Fibroblast Growth Factor 2 (Basic) (FGF2) antibody

Antigen: Fibroblast Growth Factor 2 (Basic) (FGF2)

Synonyms: BFGF, FGFB, HBGF-2, bFGF, Fgf-2, FGF2, nudt6, fgf2, MGC52602, FGF-2, bfgf, fgfb, fgf-2, xfgf2, hbgh-2, HBGF2, LOC100227798, Fgfb

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649744

Additional Information

Alternative name: FGF2

Gene ID: 2247

UniProt: P09038

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human FGF2 was used as an immunogen.

Molecular Weight: 18, 21, 24 kDA

Comments:

Background: Basic fibroblast growth factor (FGF2) is a wide-spectrum mitogenic, angiogenic, and neurotrophic factor that is expressed at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary. FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth (1). It is also an important modulator of cartilage and bone growth and differentiation (2). FGF2 consists of multiple protein isoforms produced by alternative translation from the FGF2 gene. High molecular weight FGF2 isoforms are not secreted from the cell, but are transported to the nucleus where they regulate cell growth or behavior in an intracrine fashion. 18 kDa FGF2 can be secreted to the extracellular medium where it acts as a conventional growth factor by binding to and activation of cell-surface receptors (3, 4). These protein isoforms are localized to different cellular compartments, indicating unique biological activity. FGF2 isoforms in the heart have distinct roles in many pathological circumstances in the heart including cardiac hypertrophy, ischemia-reperfusion injury, and atherosclerosis (4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100 FC: ~~ 1:10~100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: FGF2 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Cycle, Cell Structure

Restrictions: For Research Use only

Publications

Product: Montero, Okada, Tomita et al.: "Disruption of the fibroblast growth factor-2 gene results in decreased bone mass and bone formation." in: The Journal of clinical investigation, Vol. 105, Issue 8, pp. 1085-93, 2000 (PubMed).

Hamosh, Scott, Amberger et al.: "Online Mendelian Inheritance in Man (OMIM), a knowledgebase of human genes and genetic disorders." in: Nucleic acids research, Vol. 33, Issue Database issue, pp. D514-7, 2004 (PubMed).

Sørensen, Nilsen, Wiedłocha: "Functional diversity of FGF-2 isoforms by intracellular sorting." in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 28, Issue 5, pp. 504-14, 2006 (PubMed).

Liao, Bodmer, Pietras et al.: "Biological functions of the low and high molecular weight protein isoforms of fibroblast growth factor-2 in cardiovascular development and disease." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 238, Issue 2, pp. 249-64, 2009 (PubMed).