Hypoxia Inducible Factor 1, alpha Subunit Inhibitor (HIF1AN) antibody

Details for Product No. ABIN649753
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Antigen
Synonyms HIF-1a, HIF1a, hif-1a, hif1a, FIH1, 2310046M24Rik, A830014H24Rik, cb728, id:ibd5139
Reactivity
Human, Rat (Rattus)
(85), (28), (25), (15), (15), (12), (12), (2), (2)
Host
Rabbit
(55), (29)
Clonality
Monoclonal
Conjugate
Un-conjugated
(4), (4), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(72), (28), (18), (18), (11), (11), (10), (2), (1), (1)
Pubmed 3 references available
Quantity 100 µL
Options
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Request Want additional data for this product?

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Catalog No. ABIN649753
450.00 $
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues in human FIH1 was used as an immunogen.
Alternative Name FIH1
Background Factor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3).
Synonyms: HIF1AN, FIH1, Hypoxia-inducible factor 1-alpha inhibitor, Hypoxia-inducible factor asparagine hydroxylase,Factor inhibiting HIF-1
Molecular Weight 40 kDA
Gene ID 55662
UniProt Q9NWT6
Research Area Signaling, Protein Modifications, Transcription Factors, Cell Structure
Application Notes The suggested dilution is: WB: = 1:1000-5000
Comment

Background: Factor inhibiting HIF-1 (FIH1) is an Fe (II)-dependent hydroxylase enzyme that serves as an oxygen sensor in the hypoxia response pathway (1). FIH1 binds to HIF-1alpha and inhibits its transactivation function. It also binds to VHL, which too functions as a transcriptional corepressor that inhibits HIF-1alpha transactivation function (2). In addition, FIH1 regulates the Notch signaling output and plays a role in how Notch signaling modulates the hypoxic response. It negatively regulates Notch activity and accelerates myogenic differentiation (3).

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment FIH1 Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Mahon, Hirota, Semenza: "FIH-1: a novel protein that interacts with HIF-1alpha and VHL to mediate repression of HIF-1 transcriptional activity." in: Genes & development, Vol. 15, Issue 20, pp. 2675-86, 2001 (PubMed).

Lando, Peet, Gorman et al.: "FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor." in: Genes & development, Vol. 16, Issue 12, pp. 1466-71, 2002 (PubMed).

Zheng, Linke, Dias et al.: "Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 9, pp. 3368-73, 2008 (PubMed).

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