Forkhead Box O1 (FOXO1) antibody

Details for Product No. ABIN649759
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms FOXO1, FOXO1A, fkh1, fkhr, foxo1a, xFoxO1, FKH1, FKHR, AI876417, Afxh, Fkhr1, Foxo1a, Fkhr, FoxO1A, FoxO1a.1, foxo1, zgc:153388
Reactivity
Human, Rat (Rattus)
(310), (192), (165), (77), (77), (77), (61), (2), (1), (1), (1)
Host
Rabbit
(256), (47), (8), (1)
Clonality
Monoclonal
Conjugate
Un-conjugated
(6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(233), (112), (85), (72), (60), (53), (37), (4), (4), (3), (2)
Pubmed 1 reference available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 2 to 3 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN649759
450.00 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Specificity A synthetic peptide corresponding to residues surrounding Ser329 of human FKHR was used as immunogen. Anti-FKHR RabMab recognizes both phosphorylated and unphosphorylated FKHR.
Alternative Name FKHR
Background FKHR (FoxO1), a member of the FoxO subfamily of forkhead transcription factors, is an important target for insulin and growth factor signaling in the regulation of metabolism, cell cycle and proliferation, and survival in peripheral tissues (1). FKHR is regulated by insulin via Akt-dependent phosphorylation and nuclear exclusion. It has been shown that FoxO1 is induced in the early stages of adipocyte differentiation but that its activation is delayed until the end of the clonal expansion phase. Constitutively active FoxO1 prevents the differentiation of preadipocytes, while dominant-negative Foxo1 restores adipocyte differentiation of fibroblasts from insulin receptor-deficient mice (2). Conserved residue (Ser329) is a novel in-vivo phosphorylation site on FKHR. Phosphorylation of this site also decreases the ability of FKHR to stimulate gene transactivation and reduces the proportion of FKHR present in the nucleus (3)
Synonyms: FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A,Forkhead in rhabdomyosarcoma
Molecular Weight 80 kDA
Gene ID 2308
UniProt Q12778
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: = 1:500-1000, IHC: = 1:250-500
Comment

Background: FKHR (FoxO1), a member of the FoxO subfamily of forkhead transcription factors, is an important target for insulin and growth factor signaling in the regulation of metabolism, cell cycle and proliferation, and survival in peripheral tissues (1). FKHR is regulated by insulin via Akt-dependent phosphorylation and nuclear exclusion. It has been shown that FoxO1 is induced in the early stages of adipocyte differentiation but that its activation is delayed until the end of the clonal expansion phase. Constitutively active FoxO1 prevents the differentiation of preadipocytes, while dominant-negative Foxo1 restores adipocyte differentiation of fibroblasts from insulin receptor-deficient mice (2). Conserved residue (Ser329) is a novel in-vivo phosphorylation site on FKHR. Phosphorylation of this site also decreases the ability of FKHR to stimulate gene transactivation and reduces the proportion of FKHR present in the nucleus (3)

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment FKHR Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Woods, Rena, Morrice et al.: "The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site." in: The Biochemical journal, Vol. 355, Issue Pt 3, pp. 597-607, 2001 (PubMed).

Validation Images
Did you look for something else?
back to top