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Details for Product No. ABIN649759

Forkhead Box O1 (FOXO1) antibody

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Antigen
Synonyms FKH1, FKHR, FOXO1A, Afxh, Fkhr1, Foxo1a, AI876417, Fkhr, foxo1, FoxO1a.1, zgc:153388, FoxO1A, FOXO1, fkh1, fkhr, foxo1a, xFoxO1
Reactivity
»Alternatives Human, Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Pubmed 1 reference available
Catalog no. ABIN649759
Quantity 100 µL
Price
450.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Specificity A synthetic peptide corresponding to residues surrounding Ser329 of human FKHR was used as immunogen. Anti-FKHR RabMab recognizes both phosphorylated and unphosphorylated FKHR.
Alternative Name FKHR
Background FKHR (FoxO1), a member of the FoxO subfamily of forkhead transcription factors, is an important target for insulin and growth factor signaling in the regulation of metabolism, cell cycle and proliferation, and survival in peripheral tissues (1). FKHR is regulated by insulin via Akt-dependent phosphorylation and nuclear exclusion. It has been shown that FoxO1 is induced in the early stages of adipocyte differentiation but that its activation is delayed until the end of the clonal expansion phase. Constitutively active FoxO1 prevents the differentiation of preadipocytes, while dominant-negative Foxo1 restores adipocyte differentiation of fibroblasts from insulin receptor-deficient mice (2). Conserved residue (Ser329) is a novel in-vivo phosphorylation site on FKHR. Phosphorylation of this site also decreases the ability of FKHR to stimulate gene transactivation and reduces the proportion of FKHR present in the nucleus (3)
Synonyms: FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A,Forkhead in rhabdomyosarcoma
Molecular Weight 80 kDA
Gene ID 2308
UniProt Q12778
Research Area Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Signaling, Chromatin, Cell Structure
Application Notes The suggested dilution is: WB: ~~ 1:500~1000 ICC: ~~ 1:100~250 IHC: ~~ 1:250~500
Comment

Background: FKHR (FoxO1), a member of the FoxO subfamily of forkhead transcription factors, is an important target for insulin and growth factor signaling in the regulation of metabolism, cell cycle and proliferation, and survival in peripheral tissues (1). FKHR is regulated by insulin via Akt-dependent phosphorylation and nuclear exclusion. It has been shown that FoxO1 is induced in the early stages of adipocyte differentiation but that its activation is delayed until the end of the clonal expansion phase. Constitutively active FoxO1 prevents the differentiation of preadipocytes, while dominant-negative Foxo1 restores adipocyte differentiation of fibroblasts from insulin receptor-deficient mice (2). Conserved residue (Ser329) is a novel in-vivo phosphorylation site on FKHR. Phosphorylation of this site also decreases the ability of FKHR to stimulate gene transactivation and reduces the proportion of FKHR present in the nucleus (3)

Restrictions For Research Use only
Format Liquid
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Preservative Sodium azide
Storage -20 °C
Storage Comment FKHR Antibody can be stored at -20°C for up to 12 months from time of receipt.
Expiry Date 12 months
Background publications Woods, Rena, Morrice et al.: "The kinase DYRK1A phosphorylates the transcription factor FKHR at Ser329 in vitro, a novel in vivo phosphorylation site." in: The Biochemical journal, Vol. 355, Issue Pt 3, pp. 597-607, 2001 (PubMed).

Alternatives for antigen "Forkhead Box O1 (FOXO1)", type "Antibodies"
Hosts (235), (52), (9), (1)
Reactivities (294), (184), (154), (80), (80), (77), (62), (3), (2), (1), (1), (1)
Applications (218), (91), (80), (78), (60), (41), (30), (5), (5), (3), (3), (1)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6)
Epitopes (37), (34), (20), (13), (13), (12), (12), (8), (8), (8), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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