GRB2-Associated Binding Protein 2 (GAB2) antibody

Antigen: GRB2-Associated Binding Protein 2 (GAB2)

Synonyms: KIAA0571, GAB2

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Certificates: ISO 9001:2008

Catalog no.: ABIN649767

Additional Information

Alternative name: Gab2

Gene ID: 9846

UniProt: Q9UQC2

Description: Other names: GAB2, KIAA0571, GRB2-associated-binding protein 2, Growth factor receptor bound protein 2-associated protein 2,GRB2-associated binder 2,pp100

Characteristics: Rabbit Monoclonal Antibody

Specificity: A synthetic peptide corresponding to residues in human Gab2 was used as immunogen. This antibody is predicted to detect the splice isoform 2 based on sequence homology.

Molecular Weight: 97 kDA

Comments:

Background: Gab2 (Grb2-associated binder-2) is an adaptor protein involved in numerous intracellular signaling pathways via its interaction with receptor tyrosine kinases (RTKs), growth factors and cytokines. Gab2 is a substrate of various RTKs, including EGFR, insulin receptor, cytokine receptors as well as B and T cell antigen receptors, Gab2 acts as a docking protein for several SH2-containing proteins (1-3). Upon tyrosine phosphorylation, Gab2 interacts with SHP2 tyrosine phosphatase and GRB2 adaptor protein PI3-kinase, CrkL and SHC (4-5).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 FC: ~~ 1:20 ICC: ~~ 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: Gab2 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Weiss, Littman: "Signal transduction by lymphocyte antigen receptors." in: Cell, Vol. 76, Issue 2, pp. 263-74, 1994 (PubMed).

Holgado-Madruga, Moscatello, Emlet et al.: "Grb2-associated binder-1 mediates phosphatidylinositol 3-kinase activation and the promotion of cell survival by nerve growth factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 23, pp. 12419-24, 1997 (PubMed).

Nishida, Yoshida, Itoh et al.: "Gab-family adapter proteins act downstream of cytokine and growth factor receptors and T- and B-cell antigen receptors." in: Blood, Vol. 93, Issue 6, pp. 1809-16, 1999 (PubMed).

Zhao, Yu, Shen et al.: "Gab2, a new pleckstrin homology domain-containing adapter protein, acts to uncouple signaling from ERK kinase to Elk-1." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19649-54, 1999 (PubMed).