gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2) (pSer893) antibody

Antigen: gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2)

Synonyms: HG20, GPR51, GPRC3B, GABABR2, FLJ36928, HRIHFB2099, GABA(B)R2

Binding Site: pSer893

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human, Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649770

Additional Information

Alternative name: GABA (B) R2

Gene ID: 9568

UniProt: O75899

Characteristics: Rabbit Monoclonal Antibody

Specificity: A phospho specific peptide corresponding to residues surrounding serine 893 of human GABA(B) R2 was used as an immunogen. This antibody detects GABA(B) R2 phosphorylated on serine 893.

Molecular Weight: 105 kDA

Comments:

Background: GABA (gamma-aminobutyric acid) is the main inhibitory neurotransmitter in the mammalian central nervous system, where it exerts its effects through ionotropic (GABA(A/C)) receptors to produce fast synaptic inhibition and metabotropic (GABA(B)) receptors to produce slow, prolonged inhibitory signals. Results indicate that, in vivo, functional brain GABA(B) receptors may be heterodimers composed of GABA(B)R1 and GABA(B)R2 (1). Identification of a functional homomeric GABABR2 coupled to adenylyl cyclase suggests that the complexity of GABAB pharmacological data is at least in part due to the presence of more than one receptor and opens avenues for future research leading to an understanding of metabotropic GABA receptor signal transduction mechanisms (2). The activity of GABA(B) R2 is mediated by G-proteins that inhibit adenylyl cyclase activity, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipids hydrolysis (3).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:1000~10000 IP: ~~ 1:10~100 ICC: ~~ 1:100 IHC: ~~ 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: GABA(B) R2 Antibody Phospho (pS893) can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Neurology

Restrictions: For Research Use only

Publications

Product: White, Wise, Main et al.: "Heterodimerization is required for the formation of a functional GABA(B) receptor." in: Nature, Vol. 396, Issue 6712, pp. 679-82, 1999 (PubMed).