Prolactin-Induced Protein (PIP) antibody

Antigen: Prolactin-Induced Protein (PIP)

Clonality: Monoclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Certificates: ISO 9001:2008

Catalog no.: ABIN649781

Additional Information

Alternative name: GCDFP-15

Gene ID: 4436

UniProt: P12273

Characteristics: Rabbit Monoclonal IgG

Specificity: A synthetic peptide corresponding to residues in human GCDFP-15 was used as an immunogen.

Molecular Weight: 17 kDA

Comments:

Background: Gross cystic disease fluid protein (GCDFP-15), also called prolactin-inducible protein (PIP), is a single polypeptide chain with a versatile function in human reproductive and immunological systems (1). It is unique because of its small size (1). GCDFP-15 binds to CD4, exerts a potent inhibition on T lymphocyte apoptosis mediated by CD4/T-cell receptor (TCR) activation, and carries a fibronectin-specific aspartyl protease activity (2). Its ability to bind with other molecules like immunoglobulin G (IgG), actin, and zinc alpha2-glycoprotein (ZAG) shows its various biological activities (1). GCDFP-15 is expressed in benign and malignant human breast tumors and in normal exocrine organs such as sweat, salivary and lacrimal glands (1). It is up regulated by prolactin and androgens, while it is down regulated by estrogen (1). Due to its expression in breast cancer, GCDFP-15 is used as a prognostic biomarker (3, 4).

Application Details

Application Notes: The suggested dilution is: WB: ~~ 1:5000 IP: ~~ 1:30 FC: ~~ 1:30 ICC: ~~ 1 : 100~250 IHC: ~~ 1 : 100~250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Storage: GCDFP-15 Antibody can be stored at -20°C for up to 12 months from time of receipt.

Research Area: Cell Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Publications

Product: Hassan, Waheed, Yadav et al.: "Prolactin inducible protein in cancer, fertility and immunoregulation: structure, function and its clinical implications." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 3, pp. 447-59, 2009 (PubMed).

Yan, Gidley, Horton et al.: "Diagnostic utility of mammaglobin and GCDFP-15 in the identification of metastatic breast carcinoma in fluid specimens." in: Diagnostic cytopathology, Vol. 37, Issue 7, pp. 475-8, 2009 (PubMed).

Debily, Marhomy, Boulanger et al.: "A functional and regulatory network associated with PIP expression in human breast cancer." in: PLoS ONE, Vol. 4, Issue 3, pp. e4696, 2009 (PubMed).