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V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody

Antigen

V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2)
Synonyms Neu, HER2, HER-2, c-neu, Erbb-2, c-erbB2, mKIAA3023, NEU, NGL, TKR1, CD340, HER-2/neu
Binding Site
Alternatives

pTyr877
Clonality Monoclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Western Blotting (WB), Immunocytochemistry (ICC)
1 reference available
Certificates ISO 9001:2008
Catalog no. ABIN649825
Quantity 100ul
Price 507.14 $   Plus shipping costs $45.00
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Additional Information
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Alternative name HER2 (ErbB2)
Gene ID 2064
UniProt P04626
Description Other names: ERBB2, HER2, NEU, NGL, Receptor tyrosine-protein kinase erbB-2, p185erbB2,C-erbB-2,NEU proto-oncogene,Tyrosine kinase-type cell surface receptor HER2,MLN 19
Characteristics Rabbit Monoclonal Antibody
Specificity A phospho - specific peptide corresponding to residues surrounding tyrosine 877 of human HER2 was used as an immunogen. This antibody detects HER2 phosphorylated at tyrosine 877.
Molecular Weight 180 kDA
Comments

Background: HER2 / ErbB2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases (1). The kinase activity of ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins (2). Overexpression of ErbB2 is detected in almost 40% of human breast cancers (3). ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas. Autophosphorylation did not modulate receptor turnover. A Tyr----Phe substitution of ErbB-2 Tyr-877 homologous to pp60c-src Tyr-416 did not alter ErbB-2 biological and biochemical properties, thus excluding the possibility that phosphorylation of this residue, located in the kinase domain, modulates ErbB-2 gp185 catalytic function (4).

Application Details
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Application Notes The suggested dilution is: WB: ~~ 1:100000 IP: ~~ 1:50 FC: ~~ 1:20 ICC: ~~ 1:50~100
Buffer 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage HER2 (ErbB2) Antibody Phospho (pY877) can be stored at -20°C for up to 12 months from time of receipt.
Research Area Ubiquitin-related antibodies, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure, Signaling, Chromatin
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Product Klapper, Waterman, Sela et al.: "Tumor-inhibitory antibodies to HER-2/ErbB-2 may act by recruiting c-Cbl and enhancing ubiquitination of HER-2." in: Cancer research, Vol. 60, Issue 13, pp. 3384-8, 2000 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2)", type "Antibodies"
Hosts Rabbit (235), Mouse (147), Chicken (8), Rat (5), Sheep (4), Goat (1)
Reactivities Human (377), Rat (Rattus) (90), Mouse (Murine) (84), Dog (Canine) (41), Horse (Equine) (33), Cow (Bovine) (11), Pig (Porcine) (10), Chicken (6), Guinea Pig (5), Sheep (Ovine) (5), Cat (Feline) (1), Monkey (1)
Applications Western Blotting (WB) (236), Immunofluorescence (IF) (139), Immunohistochemistry (IHC) (124), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (117), ELISA (89), Immunoprecipitation (IP) (89), Flow Cytometry (FACS) (27), Immunohistochemistry (Fixed) (IHC (fx)) (23), Electrophoretic Mobility-Shift Assay (EMSA) (11), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8), Enzyme Immunoassay (EIA) (5), Functional Studies (Func) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Immunocytochemistry (ICC) (3), Cellular Assay (CA) (1), Dot Blot (Dot) (1), Fluorescence Microscopy (FM) (1), Immunoassay (IA) (1), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1), Radioimmunoassay (RIA) (1)
Conjugates Biotin (16), Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), FITC (2), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr1248 (27), pTyr877 (19), C-Term (18), Extracellular Domain (13), pTyr1112 (11), Internal Region (8), Cytoplasmic Domain (6), Tyr1248 (6), pTyr1221, pTyr1222 (6), N-Term (5), pThr686 (5), pTyr1221,pTyr1222 (5), pTyr1222,pTyr1221 (5), pTyr686 (4), AA 153-433 (3), AA 750-987 (3), non-pTyr1248 (3), AA 1240-1260 (2), AA 1243-1255 (2), Tyr1221,Tyr1222 (2), Tyr877 (2), pSer1113 (2), pTyr1139 (2), AA 1238-1255 (1), AA 1242-1255,C-Term (1), AA 146-195 (1), AA 23-419 (1), AA 23-652 (1), AA 860-880 (1), AA 968-1020 (1), Extracellular (1), Ser1112 (1), Ser877 (1), Tyr1196 (1), Tyr1221, Tyr1222 (1), Tyr1222 (1), pSer1108 (1), pSer486,pSer498,pSer632 (1), pThr1221, pThr1222 (1), pTyr1129 (1), pTyr1221 (1), pTyr1222 (1)